中文摘要：该研究首次尝试将脱氧雪腐镰刀菌烯醇（DON）的产生与禾谷镰刀菌中TRI5基因的表达联系起来，作为相互作用的环境因子（水活度（a（w））（0.95-0.98）、温度（20-30℃）和培养时间（7天-28天））的函数，以研究其在香菇中的生产机制。温度、水分活度和培养时间的变化可显著影响（P <0.01）DON产生和TRI5基因表达。当在20摄氏度和0.98 a（w）下培养21天时，观察到DON浓度达到最高（793.5 +/-27.4μg/ kg），TRI5基因表达为（2（-Delta Delta Ct）= 38.8 +/- 4.8）。进行多元回归分析，建立基于多项式方程的非线性模型，分别揭示温度，水分活度和培养时间的个体影响以及它们对DON产生和TRI5基因表达的相互作用。建立的模型进一步用于开发等高线图以预测香菇中DON产生、TRI5基因表达与贮藏条件的关系。在该商品中观察到DON产生和折叠TRI5基因表达之间呈高度显著正相关（R = 0.5534，P <0.0001）。本研究揭示的香菇中DON的产生机制将有利于开发更有效的靶向控制策略。
外文摘要：This study made the first attempt to relate the production of deoxynivalenol (DON) to the expression of TRI5 gene in Fusarium graminearum as a function of interacting environmental factors (water activity (a(w)) (0.95-0.98), temperature (20-30 degrees C) and incubation time (7 day-28 day)), so as to investigate its production mechanisms in Lentinula edodes. Changes in temperature, water activity and incubation time could significantly (P<0.01) affect DON production and TRI5 gene expression. The highest DON concentration (793.5 +/- 27.4 mu g/kg) and TRI5 gene expression (2(-Delta Delta Ct)=38.8 +/- 4.8) were observed when the cultures were incubated at 20 degrees C and 0.98 a(w) for 21 days. Multi-regression analysis was performed and nonlinear models based on polynomial equations were established to uncover the individual effects of temperature, water activity and incubation time as well as their interactions on DON production and TRI5 gene expression. The established model was further used to develop contour maps to predict the DON production and TRI5 gene expression in relation to storage conditions in L. edodes. Highly significant positive correlation between DON production and fold TRI5 gene expression (R=0.5534, P<0.0001) was observed in this commodity. The production mechanisms of DON in L. edodes revealed in the present study will be beneficial for developing more effective targeted control strategies.
外文关键词：deoxynivalenol; TRI5 gene expression; environmental conditions; model; Lentinula edodes
作者：Han, Z.; Shen, Y.; Di Mavungu, J. Diana; 等.
作者单位：Shanghai Academy of Agricultural Sciences
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：1.727
出版年份：2018
出版刊次：2
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