中文摘要：玉米赤霉烯酮（ZEN）毒性对人类而言是一个重大危险。因此，开发灵敏、精确且廉价的ZEN检测分析方法，尤其是对人血清中ZEN的检测具有重要意义。本文基于非靶诱导的核酸适配体步行者、金纳米颗粒（AuNPs）的催化反应、外显子酶III（Exo III）作为信号放大器，4-硝基苯酚作为比色剂，提出了一种用于ZEN测定的比色适配体传感器。低量的ZEN要求和信号放大是所提出的适体传感器的一些显著优点。所制备的传感策略成功用于人血清样品中的ZEN测定。
外文摘要：Zearalenone (ZEN) toxicity is a significant risk for human beings. Thus, it is of high importance to develop sensitive, precise, and inexpensive analytical methods for ZEN detection, especially in human serum. Here, a colorimetric aptasensor is presented for the determination of ZEN based on the nontarget-induced aptamer walker, catalytic reaction of gold nanoparticles (AuNPs), exonudease III (Exo III) as a signal amplifier, and 4-nitrophenol as a colorimetric agent. Low amount of ZEN requirement and signal amplification are some of the distinct advantages of the proposed aptasensor. In the absence of ZEN, the aptamer (Apt) starts walking on the AuNP surface with the help of Exo III and binds to multiple complementary strands of aptamer, leading to the change of sample color from yellow to colorless. Upon the addition of ZEN, both the Apt and complementary strand exist as single-stranded DNAs on the surface of AuNPs, resulting in less access of 4-nitrophenol to the surface of AuNPs and less catalytic performance of AuNPs. In this situation, the color of the sample remains yellow (the color of 4-nitrophenol). The presented aptasensor was capable to detect ZEN in a wide linear dynamic range, 20-80 000 ng/L, with a detection limit of 10 ng/L. The prepared sensing strategy was successfully used for ZEN determination in the human serum sample.
外文关键词：aptamer walker; zearalenone; gold nanoparticles; exonuclease III; sensor
作者：Taghdisi, Seyed Mohammad; Danesh, Noor Mohammad; Ramezani, Mohammad; 等.
作者单位：Mashhad University Medical Science
期刊名称：ACS APPLIED MATERIALS & INTERFACES
期刊影响因子：8.097
出版年份：2018
出版刊次：15
点击下载：用于玉米赤霉烯酮检测的基于非靶诱导的核酸适配体步行者、金纳米粒子和核酸外切酶辅助循环扩增的新型比色适配体传感器