中文摘要：玉米赤霉烯酮（ZEN）是由镰刀菌属产生的霉菌毒素，其是导致家畜生殖障碍的主要动物饲料污染物之一。本研究的目的是评估地衣芽孢杆菌菌株CK1的益生菌特征和ZEN去除能力。本研究评估了CK1的耐酸性、耐胆盐性、粘附性、抗致病性等益生菌特性。CK1在pH 值为2.0或3.0条件下培养3h后依然存活，在含0.3%草胆的肉汤中生长良好，对Caco-2细胞有粘附能力，并能抑制大肠杆菌O157：H7和单核细胞增生李斯特菌的生长。本研究将CK1的ZEN去除能力与矿物质霉菌毒素吸附剂，水合硅铝酸钙钠（HSCAS）和特征良好的生物霉菌毒素吸附剂鼠李糖乳杆菌（LGG）的ZEN去除能力进行比较。在37℃的含有5μgmL（-1）ZEN的磷酸盐缓冲盐水（PBS，pH7.0）中，CK1的ZEN去除率为73.0％，显著高于HSCAS和LGG（分别为45.9％和48.4％）。在2.5-8.0的pH范围内，CK1去除了高达65％的ZEN。在4到42℃的温度下，CK1除去了超过75％的ZEN。在吸附稳定性分析中，即使经过连续五轮洗涤程序，CK1除去的ZEN量也超过30％。这些发现表明CK1能显示出益生菌特性并有效地去除了ZEN。因此，CK1具有开发去除ZEN的饲料添加剂的巨大潜力。
外文摘要：Zearalenone (ZEN) is a mycotoxin produced by Fusarium species, which is one of the main animal feed contaminants causing reproductive disorders in livestock. The aim of this study was to evaluate the probiotic characteristics and ZEN removal ability of a Bacillus licheniformis strain CK1. The probiotic properties, including acidic tolerance, bile salt tolerance, adherence capability, and anti-pathogenic activities of CK1 were evaluated. CK1 survived after incubation at pH 2.0 or 3.0 for 3 h, grew well in LB broth containing 0.3% oxgall, possessed adherence capability to Caco-2 cells, and inhibited the growth of Escherichia coli O157: H7 and Listeria monocytogenes. The ZEN removal ability of CK1 was compared with a mineral mycotoxin-adsorbing agent, hydrated sodium calcium aluminosilicate (HSCAS), and a well-characterized biological mycotoxin-adsorbing agent, Lactobacillus rhamnosus GG (LGG). At 37 E C in phosphate-buffered saline (PBS, pH 7.0) containing 5 mu g mL(-1) of ZEN, the ZEN removal percentage of CK1 was 73.0%, which was significantly higher than that of HSCAS and LGG (45.9% and 48.4%, respectively). In the pH range of 2.5-8.0, CK1 removed up to 65% of ZEN. At temperatures between 4 and 42 E C, CK1 removed more than 75% of ZEN. In the adsorption stability analysis, the amounts of ZEN removed by CK1 was over 30% even after five consecutive rounds of washing procedures. These findings demonstrated that CK1 displayed probiotic characteristics and removed ZEN effectively. Therefore, CK1 has a great potential for the development of feed additive to remove ZEN.
外文关键词：-
作者：Hsu, Tsui-Chun; Yi, Ping-Jung; Lee, Ting-Yu; 等.
作者单位：National Taiwan University
期刊名称：PLOS ONE
期刊影响因子：2.766
出版年份：2018
出版刊次：4
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