中文摘要：采用先前验证过的用于测定单端孢霉烯和玉米赤霉烯酮的液相色谱-串联质谱方法（LCMS / MS）对T-2毒素（T-2）及其葡萄糖苷和乙酰基衍生物，T-2-3-葡萄糖苷（T-2-3G）和3-乙酰基-T-2（3A-T-2）进行定量分析。HT-2毒素（HT-2）及其乙酰基衍生物3-乙酰基-HT-2（3A-HT-2）也被列为目标检测化学物质。检查主食面粉（从新加坡市场收集的56个样本）中的T-2和/或HT-2及其衍生物的污染。其中，发现16种面粉被T-2和/或HT-2污染，而除了在2份黑麦面粉样本中检测到的极少量3A-T-2，没有一种被T-2-3G和3A-HT-2污染。黑麦面粉样本经常被T-2和HT-2污染。进一步分析了一些标准物质（RMs），并对玉米和小麦标准物质中的T-2-3G和3A-T-2进行了定量检测。标准物质中T-2-3G与T-2的比例似乎远低于以前研究中经常报道的脱氧雪腐镰刀菌烯-3-葡萄糖苷与脱氧雪腐镰刀菌烯醇的比例。据我们所知，本研究首次报道了主食面粉中3A-T-2的天然污染。
外文摘要：A determination method previously validated for trichothecenes and zearalenone by means of liquid chromatography-tandem mass spectrometry (LCMS/MS) was adapted for the quantification of T-2 toxin (T-2) as well as its glucoside and acetyl derivatives, T-2-3-glucoside (T-2-3G) and 3-acetyl-T-2 (3A-T-2). HT-2 toxin (HT-2) and its acetyl derivative 3-acetyl-HT-2 (3A-HT-2) were also included as the target chemicals. Staple flours (56 samples collected from the Singapore market) were examined for contamination from T-2 and/or HT-2 and their derivatives. Among them, 16 flours were found to be contaminated with T-2 and/or HT-2, whereas none was contaminated with T-2-3G and 3A-HT-2, except for trace 3A-T-2 detected in 2 rye samples. Rye flour samples were frequently contaminated with both T-2 and HT-2. Some of the reference materials (RMs) were further analyzed, and T-2-3G and 3A-T-2 were quantitatively detected in corn and wheat RMs. The ratio of T-2-3G to T-2 in the RMs seemed to be much lower than the ratio of deoxynivalenol-3-glucoside to deoxynivalenol usually reported in former studies. To the best of our knowledge, the natural contamination of 3A-T-2 in staple flour is reported here for the first time.
外文关键词：-
作者：Nakagawa, Hiroyuki; Matsuo, Yosuke; McCormick, Susan; 等.
作者单位：National Agriculture & Food Research Organization - Japan
期刊名称：JOURNAL OF AOAC INTERNATIONAL
期刊影响因子：1.087
出版年份：2018
出版刊次：3
点击下载：一种测定T-2毒素及其葡萄糖苷和乙酰基衍生物的液相色谱-串联质谱方法的开发，用于估算主食面粉中总T-2毒素的污染