中文摘要：黄曲霉毒素B1（AFB1）和玉米赤霉烯酮（ZON）由于其致癌性和雌激素作用而成为危险的霉菌毒素。为了减轻其对人类和动物的负面影响，需要成功的解毒工具。应用微生物降解霉菌毒素食品和饲料工业中提高食品安全性的有效途径。几种红球菌菌株能有效降解芳香族霉菌毒素，且其在霉菌毒素生物解毒过程中的应用是一个前景广阔的生物技术领域。本研究对42种红球菌菌株AFB1和ZON解毒能力进行了研究。通过使用配备有用于检测霉菌毒素浓度的荧光检测器的高效液相色谱仪分析样品，并使用SOS显色试验监测剩余的基因毒性。在42种红球菌菌株中，18种菌株可以消除超过90％的AFB1，并且在72小时内15种菌株基因毒性消失了（R. imtechensis JCM 13270（T），R. erythropolis JCM 3201（T），R. tukisamuensis JCM 11308（T），R. rhodnii JCM 3203（T），R. aerolatus JCM 19485（T），R. enclensis DSM 45688（T），R. lactis DSM 45625（T），R. trifolii DSM 45580（T） ），R. qingshengii DSM 45222（T），R. artemisiae DSM 45380（T），R. baikonurensis DSM 44587（T），R. globerulus JCM 7472（T），R. kroppenstedtii JCM 13011（T），R. pyridinivorans JCM 10940（T），R. corynebacterioides JCM 3376（T））。至于ZON，只有R.percolatus JCM 10087（T）能够降解超过90％的化合物并且减少70％的雌激素作用。
外文摘要：Aflatoxin B1 (AFB1) and zearalenone (ZON) are dangerous mycotoxins due to their carcinogenicity or oestrogenicity. To alleviate negative effects on humans and animals, successful detoxification tools are needed. The application of microorganisms to biodegrade mycotoxins can be an effective way in food and feed industry enhancing food safety. Several Rhodococcus strains are effective in the degradation of aromatic mycotoxins and their application in mycotoxin biodetoxification processes is a promising field of biotechnology. In this study, we investigated the AFB1 and ZON detoxification ability of 42 type strains of Rhodococcus species. Samples were analysed by high-performance liquid chromatograph equipped with fluorescence detector for mycotoxin concentration and SOS-chromotest was used for monitoring remaining genotoxicity. Out of the 42 Rhodococcus strains, 18 could eliminate more than 90% of the applied AFB1 and the genotoxicity was ceased by 15 strains in 72 h (R. imtechensis JCM 13270(T), R. erythropolis JCM 3201(T), R. tukisamuensis JCM 11308(T), R. rhodnii JCM 3203(T), R. aerolatus JCM 19485(T), R. enclensis DSM 45688(T), R. lactis DSM 45625(T), R. trifolii DSM 45580(T), R. qingshengii DSM 45222(T), R. artemisiae DSM 45380(T), R. baikonurensis DSM 44587(T), R. globerulus JCM 7472(T), R. kroppenstedtii JCM 13011(T), R. pyridinivorans JCM 10940(T), R. corynebacterioides JCM 3376(T)). In case of ZON, only R. percolatus JCM 10087(T) was able to degrade more than 90% of the compound and to reduce the oestrogenicity with 70%.
外文关键词：-
作者：Risa, Anita; Krifaton, Csilla; Kukolya, Jozsef; 等.
作者单位：Szent Istvan University
期刊名称：CURRENT MICROBIOLOGY
期刊影响因子：1.373
出版年份：2018
出版刊次：7
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