中文摘要：世界各地的各种美食中都包含鸡肉和可食用内脏。在内脏中，肝脏是埃及等一些国家食用最多的内脏之一，因为它含有丰富的蛋白质，是人类许多必需营养素的一种经济的来源。真菌毒素是由真菌产生的次级有毒代谢产物，不仅可以通过食用谷物，而且可以通过食用被真菌毒素污染的饲料喂养的动物的可食组织进入人类食物链。因此，基于快速、简便、廉价、有效、坚固且安全（QuEChERS）的萃取和气相色谱-串联质谱（GC-MS/MS）测定，本研究开发了一种快速测定鸡肝中8种单端孢菌素的方法。该方法根据欧洲指导建议成功地得到了验证，具有令人满意的真实性（范围为85.1到108.4％）和精确度（相对标准偏差<8％），定量限在0.25到0.75μg/ kg范围内。将该方法应用于从埃及市场收集的20份鸡肝样品。65％的样品显示真菌毒素污染，浓度范围为1.3至25.0μg/ kg。检测到多达四种真菌毒素，最常检测到的真菌毒素是脱氧雪腐镰刀菌烯醇。
外文摘要：Chicken meat and edible offal are included in a wide number of preparations in the worldwide cuisine. Among the offal, the liver is one of the most consumed in some countries like Egypt since it is rich in proteins and is an economical source of many essential nutrients for humans. Mycotoxins, secondary toxic metabolites produced by fungi, have the potential to enter human food chain not only with cereal consumption but also through edible tissues from animals fed with mycotoxin-contaminated feed. Accordingly, a rapid method for the determination of eight trichothecenes in chicken liver was developed based on a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) determination. The proposed method was successfully validated according to the European guideline recommendations with satisfactory trueness (recovery range from 85.1 to 108.4%), precision (relative standard deviation < 8%) and limits of quantitation in a range from 0.25 to 0.75 mu g/kg. The method was applied to twenty chicken liver samples collected from Egypt markets. 65% of samples showed mycotoxin contamination in a concentration range from 1.3 to 25.0 mu g/kg. Up to four mycotoxins were detected, being deoxynivalenol the most commonly detected mycotoxin.
外文关键词：Mycotoxins; Chicken; Liver; GC-MS/MS; Co-occurrence
作者：Mahmoud, Abdallah Fikry; Escriva, Laura; Rodriguez-Carrasco, Yelko; 等.
作者单位：University of Valencia
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY
期刊影响因子：3.129
出版年份：2018
出版刊次：-
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