中文摘要：免疫层析法（ICA）是一种前景良好的现场检测技术。尽管如此，ICA的广泛应用受到其若干缺点的阻碍，例如重复性差、灵敏度低和只能单目标检测。因此，本研究开发了一种新型的基于量子点纳米微球（QB）的多重ICA（QB-ICA），其具有多条检测线，用于同时定量检测黄曲霉毒素B-1（AFB（1））和玉米赤霉烯酮（ZEN），其中以高发光的QBs作为标记物以增强分析的灵敏度。此外，引入不受目标真菌毒素干扰的链霉抗生物素蛋白（SA）-生物素系统作为对照线的信号输出。因此，获得稳定且可靠的T / C值（测试线上的信号与对照线的信号的比率）作为定量信号。本研究提出的QB-ICA对同时检测AFB1和ZEN显示出高灵敏度，其中半数最大抑制浓度分别低至38.98 pg mL（-1）和1.23 ng mL（-1）。在10％竞争抑制浓度下，AFB1和ZEN的检测限（LOD）分别为1.65和59.15 pg mL（-1）。对AFB（1）和ZEN进行定量，一个测定内的平均回收率为81.77％至119.70％，测定间的平均回收率为94.18％至111.4％，且变异系数小于12％，从而表明提出的方法是高度准确且稳健的。这些研究结果表明，使用链霉抗生物素蛋白（SA）-生物素系统作为对照线信号输出的QB-ICA法是一种用于快速筛查霉菌毒素的优秀护理平台。
外文摘要：Immunochromatographic assay (ICA) is a promising technology for on-site detection. Nonetheless, the wide-scale application of ICA is hindered by several disadvantages, such as poor reproducibility, low sensitivity, and single-target detection. Thus, a novel quantum dot nanobead (QB)-based multiplexed ICA (QB-ICA) with multiple test lines was developed in this study for the simultaneous quantitative detection of aflatoxin B-1 (AFB(1)) and zearalenone (ZEN), where QBs with high luminescence were used as labels to enhance the analytical sensitivity of the ICA. Moreover, a streptavidin (SA)-biotin system, which was undisturbed by the target mycotoxins, was introduced as the signal output for the control line. Consequently, stable and reliable T/C values (ratios of signals on the test line to that of the control line) were obtained as quantitative signals. The proposed QB-ICA demonstrated high sensitivity for the simultaneous detection of AFB1 and ZEN, of which the half-maximal inhibitory concentrations reached as low as 38.98 pg mL(-1) and 1.23 ng mL(-1), respectively. At 10% competitive inhibition concentration, the limit detections (LOD) were 1.65 and 59.15 pg mL(-1) for AFB1 and ZEN, respectively. The average recoveries of the intra-and inter-assays ranged from 81.77% to 119.70% and from 94.18% to 111.4% for AFB(1) and ZEN quantification, respectively, and the variation coefficients were less than 12%, thereby indicating that the proposed method is highly accurate and robust. These findings suggest that QB-ICA using SA-biotin system as the signal output of control line is an excellent point-of-care platform for the rapid screening of mycotoxins.
外文关键词：Immunochromatographic assay; Multiplexed; Quantum dot nanobead; Streptavidin-biotin system
作者：Shao, Yanna; Duan, Hong; Guo, Liang; 等
作者单位：Nanchang University
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：5.123
出版年份：2018
出版刊次：-
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