中文摘要：脱氧雪腐镰刀菌烯醇（DON）是一种B型单端孢霉烯霉菌毒素，常见于侵染了镰刀菌的谷物中。在人类食物链中，世界上大多数国家的DON最大耐受水平为0.75 mg kg（-1）；在动物饲料中则为1-5 mg kg（-1），具体取决于饲料原料和/或动物物种，因为DON对人类健康和动物生产力的不利影响时间长短不同。准确分析DON及其某些真菌/细菌代谢物的能力在食品/饲料分析和研究中越来越重要。本研究使用免疫亲和方法来富集和检测DON及其三种细菌代谢物，即3-epi-DON，3-酮基-DON和deepoxy-DON（DOM-1）。优化后的富集步骤与高效液相色谱相结合可以准确且可重复地量化饲料基质中的上述代谢物（本文以青贮提取物为例）。它最大限度地减少了任何背景干扰，并为分析测定这些代谢物提供了快速且易于操作的方案。更重要的是，所提供的数据证明了起初为了在酶联免疫吸附试验（ELISA）中捕获DON而产生应用​​的单克隆抗体与三种少毒/无毒的DON代谢物交叉反应的能力。当使用所研究的抗体通过基于免疫亲和力的分析评估DON污染时，这引起了人们对解释这种交叉反应性的真正需要的担忧。
外文摘要：Deoxynivalenol (DON) is a type B trichothecene mycotoxin that is commonly detected in grains infested with Fusarium species. The maximum tolerated levels of DON in the majority of world's countries are restricted to 0.75 mg kg(-1) within the human food chain and to less than 1-5 mg kg(-1) in animal feed depending on the feed material and/or animal species due to DON's short and long-term adverse effects on human health and animal productivity. The ability to accurately analyze DON and some of its fungal/bacterial metabolites is increasingly gaining a paramount importance in food/feed analysis and research. In this study, we used the immuno-affinity approach to enrich and detect DON and three of its bacterial metabolites, namely 3-epi-DON, 3-keto-DON, and deepoxy-DON (DOM-1). The optimized enrichment step coupled with high performance liquid chromatography can accurately and reproducibly quantify the aforementioned metabolites in feed matrixes (silage extract as an example in this case). It minimizes any background interface and provides a fast and easy-to-operate protocol for the analytical determination of such metabolites. More importantly, the presented data demonstrates the ability of the utilized monoclonal antibody, generated originally to capture DON in Enzyme-Linked Immunosorbent Assays (ELISA), to cross react with three less/non-toxic DON metabolites. This raises the concerns about the genuine need to account for such cross-reactivity when DON contamination is assessed through an immuno-affinity based analyses using the investigated antibody.
外文关键词：Immuno-affinity; 3-epi-DON; 3-keto-DON; DOM-1; Cross-reactivity; HPLC
作者：Zhu, Yan; Hassan, Yousef I.; Shao, Suqin; 等
作者单位：Agriculture & Agri Food Canada
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.716
出版年份：2018
出版刊次：-
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