中文摘要：玉米赤霉烯酮（ZEN）及其作为动物组织或器官中生物标志物的衍生物的测定在霉菌毒素监测中起重要作用，并且可以促进有效的暴露评估。本研究开发并验证了液相色谱-串联质谱（LC-MS/MS）方法，用于同时对9种ZEN样真菌毒素进行定量，包括在不同猪组织（心脏、肝脏、脾脏和肌肉）中的三种葡萄糖苷酸。使用快速、简便、廉价、有效、坚固且安全（QuEChERS）的提取和净化程序提取组织样品，并通过LC-MS/MS在多反应监测（MRM）模式下进行分析。用R-2在0.916和0.999之间测定每种目标分析物的动态线性范围。六种ZEN的检测限在0.5-1ng/g的范围内，定量限在1ng/g至2ng/g之间变化。令人满意的日内和日间重复性（RSDr和RSDR均<20％）表明该方法具有良好的稳定性。9种目标分析物的回收率在70-110％之间。验证结果表明，该结合QuEChERS样品预处理的LC-MS/MS方法是有效的，适用于对猪体内的ZEN代谢物同时定量。它已被应用于本研究中的猪组织分析，也适用于霉菌毒素研究领域中的其他样本。
外文摘要：The determination of zearalenone (ZEN) and its derivatives as biomarkers in animal tissues or organs plays an important role in mycotoxin monitoring and can promote effective exposure assessment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of nine ZEN-like mycotoxins, including three glucuronides in different pig tissues (heart, liver, spleen and muscle) was developed and validated in this study. Tissue samples were extracted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and clean-up procedure, and analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. Dynamic linear ranges for each target analyte were determined with R-2 between 0.916 and 0.999. The LODs of the six ZENs were achieved in the range of 0.5-1 ng/g and the LOQs varied from 1 ng/g to 2 ng/g. The satisfying intra-day and inter-day reproducibility (both RSDr and RSDR < 20%) indicated a good stability of this method. The recoveries of the nine target analytes were in the range of 70-110%. The validation results showed that this LC-MS/MS method coupled with QuEChERS sample pretreatment is effective and suitable for the simultaneous quantitation of ZEN metabolites in pigs. It has been applied to analysis of the pig tissues in this research and can be also adapted for samples in the mycotoxin research field.
外文关键词：zearalenone; LC-MS/MS; QuEChERS; pigs
作者：Yan, Zheng; Wang, Lan; Wang, Jun; 等.
作者单位：Chinese Academy of Sciences
期刊名称：TOXINS
期刊影响因子：3.273
出版年份：2018
出版刊次：3
点击下载：使用基于QuEChERS的液相色谱-串联质谱法同时测定猪体内9种类似玉米赤霉烯酮的真菌毒素
