中文摘要：本研究开发了灵敏、快速、可重复的图像表面等离子体共振（iSPR）生物传感器测定法，用于检测小麦中的T-2毒素和T-2毒素-3-葡萄糖苷（T2-G）。在该竞争性测定中，在将二级抗体（Ab（2））与金纳米颗粒结合后使用扩增策略。用甲醇/水混合物（80：20v/v）提取小麦样品，然后用等体积的一级抗体（Ab（1））稀释以用于分析。制备基质匹配的校准曲线以确定T-2毒素和T2-G。在三个浓度的空白小麦中进行回收试验。平均回收率为86％至90％，重复性相对标准偏差（RSDr）小于6％。T-2毒素的检测限为1.2ng/mL，T2-G的检测限为0.9ng/mL，相当于它们在小麦中的水平，分别为48和36μg/ kg。开发的iSPR测定法快速、灵敏，为小麦中T-2毒素/T2-G的监测提供了足够的灵敏度。这是首次将iSPR分析法用于检测小麦中“隐藏的”T2-G的试验。
外文摘要：A sensitive, rapid, and reproducible imaging surface plasmon resonance (iSPR) biosensor assay was developed to detect T-2 toxin and T-2 toxin-3-glucoside (T2-G) in wheat. In this competitive assay, an amplification strategy was used after conjugating a secondary antibody (Ab(2)) with gold nanoparticles. Wheat samples were extracted with a methanol/water mixture (80: 20 v/v), then diluted with an equal volume of primary antibody (Ab(1)) for analysis. Matrix-matched calibration curves were prepared to determine T-2 toxin and T2-G. Recovery studies were conducted at three spiking levels in blank wheat. Mean recoveries ranged from 86 to 90%, with relative standard deviations for repeatability (RSDr) of less than 6%. Limits of detection were 1.2 ng/mL of T-2 toxin and 0.9 ng/mL of T2-G, equivalent to their levels in wheat, of 48 and 36 mu g/kg, respectively. The developed iSPR assay was rapid and provided enough sensitivity for the monitoring of T-2 toxin/T2-G in wheat. This is the first iSPR assay useful for detecting the "masked" T2-G in wheat.
外文关键词：biosensor; iSPR; gold nanoparticle; T-2 toxin; T-2 toxin-3-glucoside; wheat
作者：Hossain, Md Zakir; McCormick, Susan P.; Maragos, Chris M
作者单位：United States Department of Agriculture
期刊名称：TOXINS
期刊影响因子：3.273
出版年份：2018
出版刊次：3
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