中文摘要：本研究以改进的QuEChERS法（快速、简易、廉价、高效、坚固和安全）和液相色谱-串联质谱(LC-MS/MS)为基础，开发了一种简单、快速、灵敏、可靠的方法用于同时检测水稻、小麦、玉米、燕麦、黑麦及大麦籽粒中的黄曲霉毒素B1(AFB1)和杂色曲霉素(STC)。并用该方法成功测定了十个源于我国广东省的非加工大米样品中的AFB1和STC的含量。
外文摘要：In this study, a simple, fast, sensitive and reliable method was developed for the simultaneous determination of aflatoxin B1 (AFB1) and sterigmatocystin (STC) in rice, wheat, maize, oat, rye and barley grains based on a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). AFB1 and STC were extracted using 95% acetonitrile in water and cleaned up using N-Propylethane-1,2-diamine (PSA). The method was validated according to the SANCO/12571/2013 regulation of the European Union (EU). Recoveries at three spiked levels of 0.5, 2.5 and 5.0 μg/kg ranged from 77.7% to 119.7% and the relative standard deviations (RSDs) were between 1.3% and 12.8%. Limits of detection (LOD) were 0.03 and 0.02 μg/kg for AFB1 and STC, respectively. The limit of quantification (LOQ) was 0.5 μg/kg for both AFB1 and STC. The proposed method was successfully applied to determine AFB1 and STC levels in ten non-processed rice samples from Guangdong province in China.
外文关键词：Aflatoxin B-1；Sterigmatocystin；Grains；QuEChERS；LC-MS/MS
作者：Zhao, YR；Huang, JX；Ma, LY；Wang, FH
作者单位：China Agr Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：4
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