中文摘要：本研究结合分子印迹聚合物和晶胶的优点，致力于开发提取食品样品中黄曲霉毒素的新一代整体柱。为了达到研究目的，通过同时印迹黄曲霉B1、B2、G1和G2亚型，设计了多克隆塑性抗体。这些多克隆塑性抗体即便在竞争条件下，抗赭曲霉素A和黄曲霉毒素M的印迹效率分别为1.84和26.39。最后通过研究柱内和柱间变化测试了整体柱的可重用性、可重复性、再现性和稳健性。
外文摘要：Herein, we focused on developing a new generation of monolithic columns for extracting aflatoxin from real food samples by combining the superior features of molecularly imprinted polymers and cryogels. To accomplish this, we designed multiclonal plastic antibodies through simultaneous imprinting of aflatoxin subtypes B1, B2, G1, and G2. We applied Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and spectrofluorimetry to characterize the materials, and conducted selectivity studies using ochratoxin A and aflatoxin M1 (a metabolite of aflatoxin B1), as well as other aflatoxins, under competitive conditions. We determined optimal aflatoxin extraction conditions in terms of concentration, flow rate, temperature, and embedded particle amount as up to 25 ng/mL for each species, 0.43 mL/min, 7.0, 30 °C, and 200 mg, respectively. These multiclonal plastic antibodies showed imprinting efficiencies against ochratoxin A and aflatoxin M1 of 1.84 and 26.39, respectively, even under competitive conditions. Finally, we tested reusability, repeatability, reproducibility, and robustness of columns throughout inter- and intra-column variation studies.
外文关键词：Molecular imprinting；Multiclonal plastic antibodies；Simultaneous imprinting；Cryogels；Aflatoxin extraction；Food samples
作者：Bayram, E；Yilmaz, E；Uzun, L；Say, R；Denizli, A
作者单位：Hacettepe Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：4
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