中文摘要：与霉菌毒素不同群体或‘家族’相关的问题为人们所知已有一段时间，多年来，也了解到某些群体的霉菌毒素在商品和食品中共现。直到最近，分析商品和食品中的个别或相关毒素组，并尝试测量多组毒素，需要投入大量的时间、精力和费用。近年来，分析技术，不管是仪器密集型技术如质谱法，还是筛选技术如免疫法，都得到了长足发展，形成了多种方法联用并能检测多组霉菌毒素的新技术。尽管取得了这些进步，但常规监测多种毒素的挑战仍然存在，如协同萃取多个分析物，它们具有不同的极性和基质成份携带能力，并对结果产生影响，就与使用的检测技术（MS法或免疫法）无关。由于分析多种毒素的平台众多，具体的挑战也存在于分析平台当中。本研究表明，色谱法光学检测黄曲霉毒素在相当长的时期内保持着稳定的响应因子，假如单一信号一旦在有效的实践中显示就能得到普遍接受，就可以为减少分析负担提供可能性。不过这还需要得到更多的实验团队的验证。对免疫传感器的挑战包括传感器的可重用性及对色谱分析，包括选择有效的校准系统。本文设法进一步说明与多种毒素分析相关的挑战并阐明近期这些挑战得到了怎样的处理。
 
外文摘要：The problems associated with different groups or 'families' of mycotoxins have been known for some time, and for many years certain groups of mycotoxins have been known to co-occur in commodities and foods. Until fairly recently commodities and foods were analysed for individual toxins or groups of related toxins and attempts to measure multiple groups of toxins required significant investments in terms of time, effort, and expense. Analytical technologies using both the instrument-intensive techniques, such as mass spectrometry, and screening techniques, such as immunoassays, have progressed significantly in recent years. This has led to the proliferation of techniques capable of detecting multiple groups of mycotoxins using a variety of approaches. Despite considerable progress, the challenges for routine monitoring of multiple toxins continue. Certain of these challenges, such as the need for co-extraction of multiple analytes with widely different polarities and the potential for carry-over of matrix components that can influence the results, are independent of the analytical technique (MS or immunoassay) used. Because of the wide variety of analytical platforms used for multi-toxin analysis, there are also specific challenges that arise amongst the analytical platforms. We showed that chromatographic methods with optical detection for aflatoxins maintain stable response factors over rather long periods. This offers the potential to reduce the analytical burden, provided the use of a single signal receives general acceptance once shown in practise as working approach. This must however be verified by a larger community of laboratories. For immunosensors the arising challenges include the reusability of sensors and, for chromatography-based assays they include the selection of appropriate calibration systems. In this article we seek to further describe the challenges associated with multi-toxin analysis and articulate how such challenges have recently been addressed.
外文关键词：mycotoxins；plant toxins；liquid chromatography；mass spectrometry；determination；biosensors
作者：Stroka, J；Maragos, CM
作者单位：European Commiss
期刊名称：World Mycotoxin Journal
期刊影响因子：2.091
出版年份：2016
出版刊次：5
点击下载：分析多种霉菌毒素的挑战