中文摘要：Bt杀虫蛋白在植物中持续表达，会对非靶标食草动物提供向其不同营养级的天敌和寄生昆虫获取和传递蛋白的机会。本文在实验室和田间条件下，研究了表达Cry1Ab的转基因水稻KMD1和KMD2、及非转基因水稻Xiushui 11对非靶标捕食者Onus tantilus的影响。研究发现，与对照组相比，转基因水稻没有显著影响Onus tantilus的主要生活史特征（总若虫期、成虫寿命、产卵期、产卵量），除了KMD1捕食者的繁殖力明显低于KMD2个对照组的捕食者。酶联免疫吸附法（ELISA）的检测结果表明，在取食转基因水稻牧草虫和花粉的Onus tantilus中没有检测到Cry1Ab蛋白，而转基因水稻花粉中有Cry1Ab蛋白。对Onus tantilus丰度两年的现场监测表明，与对照组相比转基因水稻对该捕食者的种群动态、季节平均密度没有显著的不利影响。因此，表达Cry1Ab的转基因水稻（KMD1和KMD2）对Orius tantilus构成的风险可忽略不计。
外文摘要：Various rice lines have been genetically modified with genes from Bacillus thuringiensis (Bt) to continuously produce Bt insecticidal proteins against lepidopteran larvae. The Bt insecticidal protein constantly expresses in the plants to create an opportunity for non-target herbivores to acquire and convey the protein to their predators or parasitoids across trophic levels. This paper evaluates the effects of Bt rice (namely, Kemindao 1 (KMD1) and Kemindao 2 (KMD2)) expressing Cry1Ab as compared to its non-Bt control line, Xiushui 11 on non-target predator Onus tantilus (a generalist predatory anthocorid of thrips) under laboratory and field conditions. To measure several biological parameters such as total nymphal duration and fecundity of this bug, it was reared on thrips and pollens of KMD1 and KMD2 as compared to their control under laboratory conditions. By comparison with the control, Bt rice did not significantly affect main life-history characteristics (total nymphal duration, female adult longevity, oviposition period and fecundity) of this anthocorid preying on Bt rice-fed thrips along with Bt rice pollens, except that the fecundity of this predator for KMD1 was distinctly lower as compared with KMD2 or the control. Enzyme-linked immunosorbent assay (ELISA) results showed that no Cry1Ab protein was detected in this predator fed on thrips or rice pollen from Bt rice but was in Bt rice pollens. With the beat plate, plastic bag and color trap sampling methods, two-year field monitoring of O. tantilus abundance demonstrated that Bt rice had no significant detrimental effects on the population dynamics and seasonal average densities of this predatory anthocorid as compared with the control. Thus, it is suggested that growing our tested Bt rice (KMD1 and KMD2) producing Cry1Ab will pose a negligible risk to the anthocorid, O. tantilus.
外文关键词：Orius tantilus; Bt rice; Cry1Ab; risk assessment; non-target effects
作者：Raen, Akhtar Zunnu; Dang Cong; Wang Fang; 等
作者单位：Zhejiang Univ
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE
期刊影响因子：0.724
出版年份：2016
出版刊次：9
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