中文摘要：对难以转化的植物进行基因组编辑在技术上是具有挑战的，且通常会涉及转基因中间体，从而会导致监管问题。本文提出了两个简单而有效的植物基因组编辑方法，这些植物可通过瞬时表达CRISPR/Cas9 DNA或RNA的愈伤组织细胞再生。这种基于瞬时表达的基因组编辑系统对T0代非转基因和纯合小麦突变体有高效性和特异性。本文用该方法来编辑六倍体面包小麦和四倍体硬粒小麦的基因，并在没有可检测转基因的条件下，该方法也可生成突变体。本文的方法也可适用于其他植物，从而加快基础和应用植物基因组工程的研究。
外文摘要：Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. This transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the T0 generation. We demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. Our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research.
作者：Zhang, Yi; Liang, Zhen; Zong, Yuan; 等
作者单位：Chinese Acad Sci
期刊名称：NATURE COMMUNICATIONS
期刊影响因子：11.329
出版年份：2016
出版刊次：8
点击下载：通过瞬时表达CRISPR/Cas9 DNA or RNA来实现小麦基因组的高效和非转基因编辑