中文摘要：本文通过识别花椰菜花叶病毒35S（CaMV 35S）制备了一个用于转基因大豆检测的无标记电化学阻抗DNA生物传感器。在该检测系统中，作者用金纳米粒子修饰的多壁碳纳米管-还原氧化石墨烯纳米带来固定单链DNA的探针。当目标DNA通过杂交反应固定在修饰电极表面时，由于形成的双链DNA抑制电子传递过程因而阻抗信号呈增长趋势。研究发现，该传感器可有选择地从不匹配的DNA中识别互补序列，及很好地应用于转基因大豆样品的实际检测。
外文摘要：We report a simple and label-free electrochemical impedimetric DNA biosensor for genetically modified (GM) soybean detection by recognizing the Cauliflower Mosaic Virus 35S (CaMV 35S). In the detection system, gold nanoparticles decorated multiwalled carbon nanotube-reduced graphene oxide nanoribbons were employed to anchor the probe single-stranded DNA. When the target DNA fixed on the modified electrode surface via hybridizing reaction, the impedimetric signal showed a growing tendency due to the formed double-stranded DNA restraining the electron transfer process. Under optimal conditions, the increased impedimetric signal of the proposed DNA biosensor was linear with the logarithm of the target DNA concentrations in the range of 1 x 10(-16) M-5 x 10(-10) M with a low detection limit of 33 x 10(-17) M. Moreover, the biosensor possessed excellent selectivity for discriminating complementary sequences from mismatched DNA as well as satisfactorily applied for practical detection of GM soybean samples.
外文关键词：Impedimetric DNA biosensor; Multiwalled carbon nanotube-reduced graphene oxide nanoribbon; Cauliflower Mosaic Virus 35S; Genetically modified soybean detection
作者：Wang, Shanshan; Liu, Qian; Li, Henan; 等
作者单位：Jiangsu Univ
期刊名称：JOURNAL OF ELECTROANALYTICAL CHEMISTRY
期刊影响因子：2.822
出版年份：2016
出版刊次：12
点击下载：通过识别CaMV 35S启动子来制备用于转基因大豆检测的无标记电化学阻抗DNA生物传感器