中文摘要：低温胁迫会影响植物的ICE1 CBF冷响应信号通路，从而导致一系列基因的过量表达来提高抗冻性。本文将一种新型MYC-type ICE1-like基因BcICE1从大白菜中分离，并研究其在水稻抗冻性的作用。含有BcICE1基因的组成性表达在茎、叶中的转录水平比根中的转录水平高。低温、ABA和NaCl处理可显著上调BcICE1的表达水平，但脱水胁迫对其表达的影响较小。低温胁迫下，过度表达BcICE1的水稻株会增强耐冻性，因其与非转基因植物相比成活率较高、可溶性糖和脯氨酸的积累较高、电解质渗漏率和MDA水平显著下降、叶绿素含量较高。在低温胁迫下，过度表达BcICE1水稻的冷诱导基因下游的OsDREB1B、OsTPP1、J013091D15、J023041L05的表达水平明显上调。研究结果表明，BcICE1对提高水稻耐冻性有积极的作用，其中最有可能的是在低温胁迫下，BcICE1基因的相互作用上调了OsDREB1B、OsTPP1、J013091D15、J023041L05的表达水平。
外文摘要：Low temperature stress operates the ICE1 -CBF cold-responsive signal pathway of plants, which leads to the overexpression of a series of genes to enhance freezing tolerance. In this study, a novel MYC-type ICE1-like gene, BcICE1, was isolated from heading Chinese cabbage (Brassica campestris ssp. Pekinensis Lour. Olsson), and its function in freezing tolerance was characterized in rice. The expression of BcICE1 gene was constitutive with higher transcriptional levels in stems and leaves than in roots. The low temperature, ABA and NaCl treatments could significantly up-regulate BcICE1 expression levels, but dehydration stress had less effect on its expression. Under low temperature stress, the rice lines overexpressing BcICE1 gene enhanced freezing tolerance according to higher survival rate, higher accumulation of soluble sugars and proline, a remarkable decline in electrolyte leakage rate and MDA levels, and higher chlorophyll content compared with non-transgenic plants. As downstream cold-regulated genes, the expression levels of OsDREB1B, OsTPP1, J013091D15 and J023041L05 were obviously up-regulated in rice overexpressing BcICE1 under low temperature stress, suggesting BcICE1 was dependent of a CBF/DREB1 cold-responsive pathway. In short, above data showed that BcICE1 had a positive effect on improving rice freezing tolerance, which most likely result from the up-regulation of OsDREB1B, OsTPP1, J013091D15 and J023041L05 expression levels by interaction with the BcICE1 gene under low temperature stress.
外文关键词：Heading Chinese cabbage; BcICE1; CBF/DEEB1; Cold-responsive genes; Rice; Freezing tolerance
作者：Xiang, Dianjun; Chai, Yongshan; Man, Lili; 等
作者单位：Heilongjiang Acad Agr Sci
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE
期刊影响因子：2.39
出版年份：2017
出版刊次：1
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