中文摘要：可编码乙烯受体、并响应生物和非生物胁迫的PpERS1基因是从桃中克隆得到的。PpERS1的基因组DNA序列包括七个外显子、六个内含子，第一个内含子的选择性剪接可产生三种不同的PpERS转录体。另外，包括ppers1启动子的2.8-kb序列通过控制GUS报告基因的表达来进行分离和分析。缺失分析表明，PpERS1启动子中存在增强子元件。利用GUS组织化学染色对PpERS1启动子进行时空表达分析，分析结果显示在转基因番茄的整个生长阶段的所有组织中均有GUS活性。将桃和转基因番茄暴露于各种胁迫，其表达模式发生了类似的变化。研究结果表明，PpERS1基因通过乙烯受体介导的信号转导可能对胁迫响应有重要作用。PpERS1启动子的特点有助于我们对桃乙烯受体转录调控的理解。
外文摘要：The PpERS1 gene, which encodes an ethylene receptor and responds to abiotic and biotic stresses, was cloned from peach (Prunus persica L. Batsch cv Okubao). The genomic DNA sequence of PpERS1 comprises seven exons which are separated by six introns, interestingly alternative splicing of the first intron produced three different PpERS1 transcripts. In addition, a 2.8-kb sequence including the promoter of PpERS1 was isolated and analyzed by placing expressing of the GUS reporter gene under its control. Several putative cis-elements were identified in the promoter of PpERS1, including two ethylene-responsive elements (EREs), five W boxes, and four putative binding sites for MYB-type transcription factors. Deletion analysis indicated the presence of an enhancer element in the PpERS1 promoter. Temporal and spatial expression analysis of the PpERS1 promoter using histochemical GUS staining showed GUS activity in all tissues examined throughout the development of transgenic tomato plants. Exposure to various stresses caused similar changes in expression patterns in peach and transgenic tomato plants. Overall, our results suggested that PpERS1 gene might play important roles in response to multiple stresses via signal transduction mediated by ethylene receptors. The characterization of the PpERS1 promoter contributes to our understanding of the transcriptional regulation of this ethylene receptor in peach.
外文关键词：ethylene receptor; PpERS1; promoter; stress responses; transcriptional expression
作者：Wang, Bao-Quan; Liu, Ji-Hong; Gong, Xiao-Qing; 等.
作者单位：Huazhong Agr Univ
期刊名称：PLANT BIOTECHNOLOGY
期刊影响因子：6.09
出版年份：2016
出版刊次：12
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