中文摘要：玉米赤霉烯酮(ZENs)是一个主要的具有雌激素活性的霉菌毒素群体，由镰刀菌产生并给人体健康和畜牧业生产带来不良影响。本文描述了一种无标记的安培型免疫传感器，用于直接检测ZENs。该方法具有高度重复性和选择性，因而为检测这些霉菌毒素提供了灵敏而便捷的工具。
外文摘要：The family of zearalenones (ZENs) represents a major group of mycotoxins with estrogenic activity. They are produced by Fusarium fungi and cause adverse effects on human health and animal production. The authors describe here a label-free amperometric immunosensor for the direct determination of ZENs. A glassy carbon electrode (GCE) was first modified with polyethyleneimine-functionalized multi-walled carbon nanotubes. Next, gold and platinum nanoparticles (AuPt-NPs) were electro-deposited. This process strongly increased the surface area for capturing a large amount of antibodies and enhanced the electrochemical performance. In a final step, monoclonal antibody against zearalenone was orientedly immobilized on the electrode, this followed by surface blocking with BSA. The resulting biosensor was applied to the voltammetry determination of ZENs, best at a working voltage of 0.18 V (vs SCE). Under optimized conditions, the method displays a wide linear range that extends from 0.005 to 50 ng mL−1, with a limit of detection of 1.5 pg mL−1 (at an S/N ratio of 3). The assay is highly reproducible and selective, and therefore provides a sensitive and convenient tool for determination of such mycotoxins.
外文关键词：Biosensor；Electroanalysis；Mycotoxins；Electrodeposition；Gold nanoparticles；Platinum nanoparticles；Cyclic voltammetry；Differential pulse voltammetry；Food analysis
作者：Liu, N；Nie, DX；Tan, YL；Zhao, ZY；Liao, YC；Wang, H；Sun, CP；Wu, AB
作者单位：Chinese Acad Sci
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：1
点击下载：将抗体定向固定在用多壁碳纳米管和纳米AuPt改进的玻碳电极上制成超灵敏安培型免疫传感器用于检测玉米赤霉烯酮