中文摘要：黄曲霉毒素，由普遍存在于加利福尼亚杏仁和开心果园中的镰刀菌产生的毒性代谢物，受其污染，每年造成数百万美元的产品损失。当前黄曲霉毒素的检测，依赖于破坏性的、昂贵而费时的实验室方法。为了寻求一种替代方法，从检测普通真菌的生长入手，本研究用静态SPME和动态针式SPE，采集了各种湿度条件的杏仁挥发组分的排放物，并用台式和便携式GC-MS分析。结果发现尽管便携式系统比台式系统检测到的挥发物更少，但二者都解决了湿度处理并确定了在极低的水活度水平下潜在的真菌生物标志物。这种解决湿度水平的能力提示萌发的真菌孢子挥发性成分可用于开发一种检测真菌生长的预警、无损的便携系统。
外文摘要：Contamination by aflatoxin, a toxic metabolite produced by Aspergillus fungi ubiquitous in California almond and pistachio orchards, results in millions of dollars of lost product annually. Current detection of aflatoxin relies on destructive, expensive, and time-intensive laboratory-based methods. To explore an alternative method for the detection of general fungal growth, volatile emission profiles of almonds at varying humidities were sampled using both static and dynamic needle-trap SPE followed by benchtop and portable GC-MS analysis. Despite the portable SPE/GC-MS system detecting fewer volatiles than the benchtop system, both systems resolved humidity treatments and identified potential fungal biomarkers at extremely low water activity levels. This ability to resolve humidity levels suggests that volatile profiles from germinating fungal spores could be used to create an early warning, nondestructive, portable detection system of fungal growth.
外文关键词：aflatoxin；fungal detection；signaling volatiles；spore；water activity
作者：Beck, JJ；Willett, DS；Gee, WS；Mahoney, NE；Higbee, BS
作者单位：ARS
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2016
出版刊次：49
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