中文摘要：本研究开发了一种高效液相色谱结合免疫亲和净化的方法用于同时测定谷物和饲料中的脱氧雪腐镰刀菌烯醇（DON）、雪腐镰刀菌烯醇(NIV)和3-乙酰脱氧雪腐镰刀菌烯醇(3AcDON)。本方法检测DON、3AcDON和NIV的定量限分别为11.0、27.6和13.5 ng/g。用HPLC法分析了从韩国收集的300谷物和饲料样品，发现DON和NIV的发病率在玉米中分别为22.5和7.5%，在水稻中分别为4.0和54.0%，而在谷物混合粉中则分别为62.0和24.0%；饲料主要受DON污染（98.1%），浓度范围为32.8–950.25 ng/g，平均浓度353.32 ng/g。谷物中DON含量不超过韩国食品药品管理局设定的最大限值(1mg/kg)。
外文摘要：An HPLC method combined with immunoaffinity clean-up was developed for the simultaneous analysis of deoxynivalenol (DON), nivalenol (NIV), and 3-acetyldeoxynivalenol (3AcDON) in grains and feeds. The limits of quantification of the method for DON, 3AcDON, and NIV were 11.0, 27.6, and 13.5 ng/g, respectively. HPLC analyses of 300 samples of grains and feeds collected in South Korea showed that the incidence of DON and NIV in corn was 22.5 and 7.5%, while that in rice was 4.0 and 54.0%, respectively. The incidence of DON and NIV in mixed grain powder was 62.0 and 24.0%, respectively. Our study also showed that animal feeds were mainly contaminated with DON (98.1%) in the range of 32.8–950.25 ng/g with the mean concentration of 353.32 ng/g. The levels of DON in grains did not exceed the maximum allowable limit (1 mg/kg) set by the Korean Food and Drug Administration.
外文关键词：3-Acetyldeoxynivalenol；Deoxynivalenol；Feed；Grain；Nivalenol
作者：Kim, DH；Hong, SY；Jeon, MH；An, JM；Kim, SY；Kim, HY；Yoon, BR；Chung, SH
作者单位：Natl Agr Prod Qual Management Serv
期刊名称：APPLIED BIOLOGICAL CHEMISTRY
期刊影响因子：0.655
出版年份：2016
出版刊次：6
点击下载：用高效液相色谱-光电二极管阵列检测器同时测定韩国谷物和饲料中的脱氧雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇和雪腐镰刀菌烯醇