中文摘要：跟踪筛选是一种鉴别真菌生物活性物质的重要方法。本研究运用快速数字延时显微镜法评估镰刀菌次生代谢物的抗真菌性能。本文测试了抗生素Y、黄色镰刀菌素、白僵菌素、恩链孢菌素和镰刀菌酸培养6小时后的抗菌作用。然后运用该系统跟踪筛选通过高效液相色谱法分离的来自14种不同镰刀菌的提取物。筛选结果发现包含红色颜料的黄色镰刀菌素和比卡菌素能抗乳酸菌和双歧杆菌。黄色镰刀菌素抗嗜酸乳杆菌的IC50为8µM，抗短双歧杆菌的IC50为64µM。黄色镰刀菌素只对益生菌有影响，由此推测其只影响促进肠道系统健康的细菌。
外文摘要：Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains of Lactobacillus and Bifidobacterium. The IC50 for aurofusarin against Lactobacillus acidophilus was 8 µM, and against Bifidobacterium breve it was 64 µM. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.
外文关键词：Fusarium；mycotoxins；secondary metabolites；bioactivity；polyketides；pigments；antibiotics；bio-guided assays；antibacterial；aurofusarin
作者：Sondergaard, TE；Fredborg, M；Christensen, AMO；Damsgaard, SK；Kramer, NF；Giese, H；Sorensen, JL
作者单位：Aalborg Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：12
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