串珠镰刀菌和禾谷镰刀菌引发的玉米穗腐病导致玉米产量下降和籽粒污染。为了绘制QTL图来抵抗穗腐病，用250个单核苷酸多态性标记对F5作图群体（包括由 LP4637 (中抗)和L4674 (敏感)杂交、随机自交获得的298个重组自交系）进行基因分型，并利用串珠镰刀菌和禾谷镰刀菌孢子悬浮液获得其接种菌丝两年后的疾病严重程度的表型。研究获得了4个对镰刀菌和环境有作用的QTL，这表明 LP4637是一种针对镰刀菌跨环境稳定性的广谱抗源。本研究结果与之前从北美、非洲、欧洲和中国抗源的相同染色体区域获得的穗腐病抗性QTL相一致。
外文摘要：
Fusarium verticillioides and F. graminearum cause ear rots in maize (Zea mays L.) that reduce yield and contaminate the grain with mycotoxins produced by the fungi. To map QTLs for resistance to these ear rots, a F5 mapping population, consisting of 298 recombinant inbreds obtained by randomly selfing of the cross between LP4637 (moderately resistant) and L4674 (susceptible), was genotyped with 250 single nucleotide polymorphism markers and phenotyped 2 years for disease severity after silk inoculation with conidial suspensions of F. verticillioides and F. graminearum. Four QTLs were mapped in chromosomes 2, 3 and 5, bins 2.03, 3.05, 3.07 and 5.07, explaining ranges of 11.2–11.8, 3.4–5.1, 6.2–7.6 and 3.8–5.0 of phenotypic variances (%), respectively, depending on year and fungus. Additive effects of each QTL ranged from 5.0 to 11.9 % of ear area covered by mold and no epistatic interactions were observed. The four QTLs were effective for both Fusarium species and environments indicating that LP4637 is a source of broad resistance to Fusarium stable across environments. These results are consistent with previous research reporting QTLs for ear rot resistance in the same chromosome regions from sources of resistance growing in North America, Africa, Europe and China.
外文关键词：Maize；Gibberella ear rot； Fusarium ear rot；Quantitative trait loci Inst Nacl Tecnol Agr；Argentina
作者：Carolina M.; Fernandez, Mariana; Silvina Oviedo, Maria; Presello, Daniel A.
作者单位：Inst Nacl Tecnol Agr,Argentina
期刊名称：EUPHYTICA
期刊影响因子：1.618
出版年份：2016
出版刊次：10
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