中文摘要：磁性固相萃取（m-SPE）是一种方便、快捷、简单、有效的样品制备方法。本研究中，创新性的利用以磁性多壁碳纳米管（m-MWCNTs）作为吸附剂的m-SPE方法从薏苡仁中提取A型单端孢霉烯族毒素，包括T-2毒素、HT-2毒素、蛇形菌素（DAS）、新茄镰孢菌醇（NEO）等。该方法的分析性能成功的应用于薏苡仁样品评价。本研究提供了利用m-SPE方法和超高效液相色谱-串联质谱法测定薏苡仁中A类单端孢霉烯族毒素抽象流程图。
外文摘要：Magnetic solid-phase extraction (m-SPE) is a promising sample preparation approach due to its convenience, speed, and simplicity. For the first time, a rapid and reliable m-SPE approach using magnetic multi-walled carbon nanotubes (m-MWCNTs) as the adsorbent was proposed for purification of type A trichothecenes including T-2 toxins (T2), HT-2 toxins (HT-2), diacetoxyscirpenol (DAS), and neosolaniol (NEO) in coix seed. The m-MWCNTs were synthesized by assembling the magnetic nanoparticles (Fe3O4) with MWCNTs by sonication through an aggregation wrap mechanism, and characterized by transmission electron microscope. Several key parameters affecting the performance of the procedure were extensively investigated including extraction solutions, desorption solvents, and m-MWCNT amounts. Under the optimal sample preparation conditions followed by analysis with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), high sensitivity (limit of quantification in the range of 0.3-1.5 μg kg-1), good linearity (R 2 > 0.99), satisfactory recovery (73.6-90.6 %), and acceptable precision (≤2.5 %) were obtained. The analytical performance of the developed method has also been successfully evaluated in real coix seed samples. Graphical Abstract Flow chart of determination of type A trichothecenes in coix seed by magnetic solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry.
中文关键词：磁性固相萃取；磁性多壁碳纳米管；超高效液相色谱-串联质谱；A型单端孢霉烯族毒素；薏苡仁
外文关键词：Magnetic solid-phase extraction；Magnetic multi-walled carbon nanotubes；Ultra-high performance liquid chromatography-tandem mass spectrometry；Type A trichothecenes；Coix seed
作者：M Dong；W Si；W Wang；等
作者单位：上海市农业科学院
期刊名称：Analytical & Bioanalytical Chemistry
期刊影响因子：3.125
出版年份：2016
出版刊次：24
点击下载：利用以磁性多壁碳纳米管（m-MWCNTs）作为吸附剂的m-SPE方法和超高效液相色谱-串联质谱法一起测定薏苡仁中A型单端孢霉烯族毒素
