中文摘要：黄曲霉毒素B1容易引发多种动物健康问题，但对鸡胸腺和法氏囊的影响尚不明确。本研究旨在探讨饲用AFB1的鸡胸腺和法氏囊细胞的凋亡情况。结果显示：食用AFB1后，鸡胸腺和法氏囊的细胞凋亡比例和Caspase-3蛋白表达均增加；同时，鸡胸腺的Bax蛋白表达增加，而Bcl-2蛋白表达下降；但鸡法氏囊的Bax和Bcl-2蛋白表达未见变化。这证实了AFB1会造成鸡胸腺和法氏囊细胞凋亡加剧和Caspase-3蛋白表达异常等不良影响。
外文摘要：Aflatoxin B1 is known as a mycotoxin that develops various health problems of animals, the effects of AFB1 on thymus and bursa of Fabricius in chickens are not clear. The objective of this study was to investigate the apoptosis of thymus and bursa of Fabricius in broilers fed with AFB1. Two hundred Avian broilers were randomly divided into four groups of 50 each, namely control group and three AFB1 groups fed with 0.15 mg, 0.3 mg, and 0.6 mg AFB1/kg diet, respectively. In this study, flow cytometer and immunohistochemical approaches were used to determine the percentage of apoptotic cells and the expression of Bax, Bcl-2, and Caspase-3. The results showed that consumption of AFB1 diets results in increased percentage of apoptotic cells and increased expression of Caspase-3 in both thymus and bursa of Fabricius. The expression of Bax was increased and the expression of Bcl-2 was decreased in the thymus, but no significant changes in Bax and Bcl-2 expression were observed in the bursa of Fabricius when broilers fed with AFB1. These findings suggest that adverse effects of AFB1 on thymus and bursa of Fabricius in broilers were confirmed by increased apoptotic cells and abnormal expression of Caspase-3.
外文关键词：apoptosis；thymus；bursa of fabricius；aflatoxin B1；Bax；Bcl-2；Caspase-3
作者：Xi Peng；Kejie Chen；Jin Chen；等
作者单位：四川农业大学
期刊名称：Environmental Toxicology
期刊影响因子：2.868
出版年份：2016
出版刊次：9
点击下载：黄曲霉毒素B1会影响肉鸡胸腺和法氏囊中Bax, Bcl-2, and Caspase-3蛋白表达和凋亡