中文摘要：本研究提出一种鲜奶中黄曲霉毒素M1（AFM1）检测的样本预处理方法，该方法具有快速、方便、廉价、有效、耐用和安全（QuEChERS）特点，可以代替传统的免疫亲和柱净化法（IAC）。该方法可以解决牛奶基质的复杂性问题，并且适用于最终的超高效液相色谱-串联质谱法 (UHPLC–MS/MS)检测方法。该方法还适合同时提取牛奶中农药残留和其他污染物。
外文摘要：A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.
外文关键词：LC–MS/MS；QuEChERS；Mycotoxins；Pesticides；Milk
作者：Nicolás Michlig；María Rosa Repetti；Carolina Chiericatti；等
作者单位：National University of Littoral Experimental Station Rafaela
期刊名称：Chromatographia
期刊影响因子：1.332
出版年份：2016
出版刊次：17-18
点击下载：用于UHPLC-MS/MS法检测鲜奶中黄曲霉毒素 M1的多类亲和样品预处理技术研究