中文摘要：本研究首创了一种利用一个单井微孔板同时定量检测三种物质的敏感性工具。该研发技术基于一种高度敏感的标签，该标签是利用胶体量子点合成硅壳(QD@SiO2)的衍生物。实验中，通过在一个单井微孔板中共固定化三种特殊的抗体（抗脱氧雪腐镰刀菌烯醇、抗玉米赤霉烯酮和抗黄曲霉毒素B1）多重免疫吸附实现了对谷物中三种霉菌毒素（脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、黄曲霉毒素B1）的筛选。并结合液相色谱串联质谱（LC-MS/MS）进行对该实验结果进行了验证。
外文摘要：A sensitive tool for simultaneous quantitative determination of three analytes in one single well of a microtiter plate is shown for the first time. The developed technique is based on use of colloidal quantum dot enrobed into a silica shell (QD@SiO2) derivatives as a highly responsive label. Silica-coated quantum dots were prepared and subsequently modified via the co-hydrolysis with tetraethylorthosilicate (TEOS) and various organosilane reagents. Different surface modification schemes were compared in terms of applicability of the obtained particles for the multiplex immunoassay, e.g. stability and simplicity of their conjugation with biomolecules. As model system a multiplex immunosorbent assay for screening of three mycotoxins (deoxynivalenol, zearalenone and aflatoxin B1) in cereal-based products was realized via a co-immobilization of three different specific antibodies (anti- deoxynivalenol, anti-zearalenone and anti-aflatoxin B1) in one single well of a microtiter plate. Mycotoxins were simultaneously determined by labelling their conjugates with QD@SiO2 emitting in different parts of the visible spectrum. The limits of detection for the simultaneous determination were 6.1 and 5.3, 5.4 and 4.1, and 2.6 and 1.9 µg kg−1 for deoxynivalenol, zearalenone and aflatoxin B1 in maize and wheat, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.
外文关键词：Multiplex；Immunoassay；Quantum dots；QD@SiO2；Mycotoxins；Rapid test
作者：Natalia V. Beloglazovaa；Astrid Fouberta；Anna Gordienkob；等
作者单位：比利时根特大学
期刊名称：Talanta
期刊影响因子：4.035
出版年份：2016
出版刊次：11
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