中文摘要：作者研发了一种基于Misens和HPLC的新型实验室芯片生物传感器，用于检测黄曲霉毒素。经实验验证，该传感器可以对食品中的黄曲霉毒素进行实时和现场检测，具有快速、灵敏、全自动化和小型化特点，将对食品行业产生巨大的经济影响。
外文摘要：Polymers were synthesized and utilized for aflatoxin detection coupled with a novel lab-on-a-chip biosensor: MiSens and high performance liquid chromatography (HPLC). Non-imprinted polymers (NIPs) were preferred to be designed and used due to the toxic nature of aflatoxin template and also to avoid difficult clean-up protocols. Towards an innovative miniaturized automated system, a novel biochip has been designed that consists of 6 working electrodes (1 mm diameter) with shared reference and counter electrodes. The aflatoxin detection has been achieved by a competition immunoassay that has been performed using the new biochips and the automated MiSens electrochemical biosensor device. For the assay, aflatoxin antibody has been captured on the Protein A immobilized electrode. Subsequently the sample and the enzyme-aflatoxin conjugate mixture has been injected to the electrode surfaces. The final injection of the enzyme substrate results in an amperometric signal. The sensor assays for aflatoxin B1 (AFB1) in different matrices were also performed using enzyme link immunosorbent assay (ELISA) and HPLC for confirmation. High recovery was successfully achieved in spiked wheat samples using NIP coupled HPLC and NIP coupled MiSens biosensor [2 ppb of aflatoxin was determined as 1.86 ppb (93% recovery), 1.73 ppb (86.5% recovery), 1.96 ppb (98% recovery) and 1.88 ppb (94.0% recovery) for immunoaffinity column (IAC)-HPLC, NIP-HPLC, Supel™ Tox SPE Cartridges (SUP)-HPLC and NIP-MiSens, respectively]. Aflatoxin detection in fig samples were also investigated with MiSens biosensor and the results were compared with HPLC method. The new biosensor allows real-time and on-site detection of AFB1 in foods with a rapid, sensitive, fully automated and miniaturized system and expected to have an immense economic impact for food industry.
外文关键词：Aflatoxin；Real-time electrochemical profiling；Amperometry；MiSens biosensor；ELISA；HPLC
作者：Y?ld?z Uludag；Elif Esen；Guzin Kokturk；等
作者单位：土耳其科学技术研究委员会
期刊名称：Talanta
期刊影响因子：4.035
出版年份：2016
出版刊次：11
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