中文摘要：本研究详细报道了一种从T-2毒素中人工合成的A型单端孢完全抗原（3-Ac-NEOS）的过程和方法，并验证了该完全抗原的优越性。该研究为所有A型单端孢霉烯族毒素类抗体的制备和酶联免疫吸附测定试剂盒的研制奠定了良好的基础。
外文摘要：We report the artificial synthesis from T-2 toxin of a type A trichothecenes complete antigen. First, 3-Ac-T-2 was made from T-2 following acetylation. Then 3-Ac-NEOS as a T-2 skeleton structure was synthesized by enzymolysis and identified by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance. Next, a hapten (3-Ac-NEOS-HS) was formed by modifying the C8 position of 3-Ac-NEOS using succinic anhydride method. 3-Ac-NEOS-HS-BSA/-OVA were prepared by conjugating hapten with bovine serum albumin (BSA) or ovalbumin (OVA) by carbodiimide method and identified by UV spectroscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Results showed that conjugation ratio of 3-Ac-NEOS-HS to BSA was 8.76: 1 and OVA 7.24: 1, indicating 3-Ac-NEOS-HS-BSA as a complete antigen was better. Next we used it to immunize rabbits and obtained a 1:64,000 antibody titre. In conclusion, a type A trichothecenes complete antigen was successfully synthesized, which was the foundation for antibody preparation and enzyme-linked immunosorbent assay kit development for all type A trichothecenes (parent + modified/masked type A trichothecenes).
外文关键词：Type A trichothecenes；T-2 toxin；3-Ac-NEOS；skeleton；complete antigen
作者：Pengli Lu；Yaling Wang；Yapei Wang；等
作者单位：广东海洋大学
期刊名称：Food and Agricultural Immunology
期刊影响因子：1.548
出版年份：2016
出版刊次：6
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