中文摘要：本研究中，利用基于便携式倏逝波的光学生物传感平台（EOBP）设计了一个灵敏检测乳制品中黄曲霉毒素M-1（AFM1）的免疫传感方法，该方法简单、低成本、可重复、易操作。试验表明，该方法可以快速、灵敏、有效的检测乳制品中的AFM1，并且无需经过复杂的样品预处理过程。
外文摘要：Aflatoxin M1 (AFM1), a highly toxic secondary metabolite, is present in a wide range of dairy products. In this study, we designed a simple, low-cost, reusable, and easy-to-operate immunosensing method for ultrasensitive detection of AFM1 in dairy products by using a portable evanescent wave-based optofluidic biosensing platform (EOBP). The developed method provides the minimum detection limit of 5 ng/L, which is below the most restrictive standard imposed by the current regulations for AFM1 in dairy products. The effect of several organic solvents, such as methanol, acetone, and acetonitrile, on the binding reaction of antibody-antigen in heterogeneous and homogeneous solutions was evaluated. Although the effect of organic solvents on the homogeneous binding reaction between antibody and antigen is more significant than that of heterogeneous binding reaction between antibody in solution and antigen immobilized onto the sensor surface, the fluorescence signal detected by EOBP is linearly dependent on AFM1 concentration. Therefore, AFM1 can be directly quantified even if the samples contain a certain organic solvent concentration. The robustness and stability of AFM1-ovalbumin conjugate allow the regeneration of modified biosensor surface for more than 200 times, thereby achieving a cost-effective and reliable AFM1 determination. The proposed method provides a rapid, ultrasensitive, and reliable AFM1 determination in dairy products without complicated sample pretreatment process.
外文关键词：Aflatoxin M1；Dairy product；Evanescent wave immunosensor；Optofluidics
作者：Xuening Lou；Anna Zhu；Hongliang Wang；等
作者单位：中国人民大学
期刊名称：Analytica Chimica Acta
期刊影响因子：4.712
出版年份：2016
出版刊次：10
点击下载：一种基于微流体的直接而灵敏检测乳制品中的黄曲霉毒素M-1的免疫测定方法