中文摘要：本研究建立了一种利用月桂醇聚氧乙烯醚盐双水相体系和高效液相色谱法同步检测环境水样和动物源食品（如牛奶、蛋品等）中磺胺嘧啶和磺胺二甲基嘧啶的方法。结果表明，样品中磺胺嘧啶和磺胺二甲基嘧啶的提取和富集效率分别受到盐的类型、盐浓度、POELE10
外文摘要：Polyoxyethylene lauryl ether (POELE10)-Na2C4H4O6 aqueous two-phase extraction system (ATPES) is a novel and green pretreatment technique to trace samples. ATPES coupled with high-performance liquid chromatography (HPLC) is used to analyze synchronously sulfadiazine (SDZ) and sulfamethazine (SMT) in animal by-products (i.e., egg and milk) and environmental water sample. It was found that the extraction efficiency (E%) and the enrichment factor (F) of SDZ and SMT were influenced by the types of salts, the concentration of salt, the concentration of POELE10 and the temperature. The orthogonal experimental design (OED) was adopted in the multi-factor experiment to determine the optimized conditions. The final optimal condition was as following: the concentration of POELE10 is 0.027 g mL(-1), the concentration of Na2C4H4O6 is 0.180 g mL(-1) and the temperature is 35 degrees C. This POELE10-Na2C4H4O6 ATPS was applied to separate and enrich SDZ and SMT in real samples (i.e., water, egg and milk) under the optimal conditions, and it was found that the recovery of SDZ and SMT was 96.20-99.52% with RSD of 0.35-3.41%. The limit of detection (LOD) of this method for the SDZ and SMT in spiked samples was 2.52-3.64 pg mL(-1), and the limit of quantitation (LOQ) of this method for the SDZ and SMT in spiked samples was 8.41-12.15 pg mL(-1).
外文关键词：Aqueous two-phase system； Polyoxyethylene lauryl ether；Separation；Sulfadiazine；Sulfamethazine
作者：Lu, Y； Cong, B；Tan, ZJ；Yan, YS
作者单位：吉林师范大学
期刊名称：ACADEMIC PRESS INC ELSEVIER SCIENCE
期刊影响因子：3.13
出版年份：2016
出版刊次：11
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