中文摘要：由禾谷镰孢菌引发的赤霉病是小麦作物中的毁灭性疾病。依靠宿主抗性来减少病害发生是最有效的策略之一。海盐种 (HYZ) 是中国的一个地方小麦品种，该品种对在麦穗中传播赤霉病 (II型抗性)具有较高的抗性。为了确定海盐种的QTLs位置以及识别与抗赤霉病QTLs紧密相关的标记，从一个由海盐种和Wheaton (易感染赤霉病的小麦品种)杂交得到的杂交小麦品种中得到172个RILs ，并利用源于GBS的SSRs和SNPs对这些RILs进行基因分型，并在三次温室试验中评估了每一穗的PSSs。最终，在海盐种中识别出6个II型抗性QTLs，表明多个小QTLs结合可形成高水平的小麦赤霉病抗性。本研究还发现对小麦赤霉病抗性有最大影响的QTL位于染色体臂5AS，海盐种的另外5个QTLs位于染色体6B, 7D, 3B, 4B和 4D。另外，Wheaton中的2个QTLs位于染色体2B。与位于染色体 5A, 6B和2B的QTLs有关的关键SNPs被转换成了KASP，这可以用于小麦QTLs的MAS。
外文摘要：Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a devastating disease in wheat (Triticum aestivum L.). Use of host resistance is one of the most effective strategies to minimize the disease damage. Haiyanzhong (HYZ) is a Chinese wheat landrace that shows a high level of resistance to FHB spread within a spike (type II resistance). To map the quantitative trait loci (QTLs) in HYZ and identify markers tightly linked to the QTLs for FHB resistance, a population of 172 recombinant inbred lines (RILs) from a cross between HYZ and Wheaton (FHB susceptible) was genotyped using simple sequence repeats (SSRs) and single-nucleotide polymorphisms (SNPs) derived from genotyping-by-sequencing (GBS), and evaluated for percentage of symptomatic spikelets (PSSs) per spike in three greenhouse experiments. Six QTLs for type II resistance were identified in HYZ, indicating that multiple minor QTLs together can provide a high level of FHB resistance in wheat. The QTL with the largest effect on FHB resistance was mapped on the chromosome arm 5AS, and the other five from HYZ were mapped on the chromosomes 6B, 7D, 3B, 4B and 4D. In addition, two QTLs from Wheaton were mapped on 2B. Critical SNPs linked to the QTLs on chromosomes 5A, 6B, and 2B were converted into KBioscience competitive allele-specific PCR (KASP) assays, which can be used for marker-assisted selection (MAS) to pyramid these QTLs in wheat.
作者：Cai J；Wang S；Li T；等
作者单位：Kansas State University
期刊名称：PLoS One
期刊影响因子：3.057
出版年份：2016
出版刊次：9
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