中文摘要：本研究尝试应用不同的QuEChERS法对样本进行预处理，并采用LC/MS/MS技术检测糙米和稻秆中三种除草剂（苄嘧磺隆，和四唑嘧磺隆、甲基磺草酮）及其代谢物(MNBA)的残留水平。试验结果表明：移除PSA吸附剂的QuEChERS方法可使提取效率提高；三种除草剂强化水平的回收率均在78~114.5之间，且所有除草剂的定量限均低于韩国食品与药品安全部制定的最大残留（MRL）值。田间试验表明，推荐剂量或双倍剂量的除草剂对水稻生产是安全的，试验水稻110天收割时未检测到除草剂残留。
外文摘要：A single-run analytical method was developed to analyze the three herbicides azimsulfuron, bensulfuron-methyl, and mesotrione and its metabolite (4-methylsulfonyl-2-nitrobenzoic acid (MNBA)) in brown rice and rice straw using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Samples extracted using various versions of Quick, Easy, Cheap, Effective, Rugged, and Safe 'QuEChERS' (original unbuffered, acetate (AOAC), and citrate (EN) buffered) methods gave poor recoveries of all the tested analytes in both matrices. The extraction efficiency was improved when primary-secondary amine (PSA) sorbent was removed from the purification step, with the best recovery being achieved for EN-QuEChERS, which was subsequently used throughout the study. Overall, a determination coefficients (R-2) >= 0.995 was achieved at matrix-matched calibration curves at various concentration ranges. The recovery rates at three fortification levels (limit of quantification (LOQ), 1/2 maximum residue limit (1/2MRL), and MRL) ranged from 78 to 114.5, with relative standard deviations (RSDs) < 18% for all the tested analytes in both matrices. The LOQs for all herbicides were lower than the MRL set by the Ministry of Food and Drug Safety (MFDS), Republic of Korea. Field trials with the recommended, or double the recommended dose, revealed that the herbicides can safely be applied to rice, as no residues were detected in the harvested samples at 110 days. (C) 2016 Elsevier Ltd. All rights reserved.
外文关键词：Herbicides；Rice；Rice straw；QuEChERS；LC/MS/MS；Residues
作者：Young-Jun Lee；Md. Musfiqur Rahman； A.M. Abd El-Aty
作者单位：Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2016
出版刊次：5
点击下载：应用多种QuEChERS前处理方法和LC/MS/MS技术检测糙米和稻秆中三种除草剂及代谢物的残留水平