中文摘要：黄曲霉毒素是严重的致癌物和免疫抑制剂。高水平黄曲霉毒素的严重暴露会导致黄曲霉毒素中毒，可以导致肝脏衰竭而快速死亡。益生菌的免疫调节作用为得天然食品脱毒提供了良好的前景。本研究旨在探讨酸奶发酵剂存在与否的情况下，嗜酸乳杆菌strainLA-5去除AFM1的能力，并与酸奶发酵剂（108 CFU ml-1）去除AFM1的能力进行对照。酸奶在4℃贮存了21天后，作者对不同浓度的黄曲霉毒素(0.1, 0.5 and 0.75 µg L-1)中的AFM1解毒情况进行了评估。利用ELISA方法检测了AFM1的残留量。与对照组相比，嗜酸乳杆菌结合酸奶发酵剂或者单独的嗜酸乳杆菌都能显著去除AFM1(P≤ 0.05)。结果表明，增加酸奶样品中AFM1初始浓度以及增加贮存时间都会影响AFM1结合的能力。
外文摘要：Aflatoxins are potent carcinogenic and immunosuppressive agents. Acute exposure to high level of aflatoxins leads to aflatoxicosis, which cause rapid death due to liver failure. Immune modulating effects of probiotic bacteria have good prospects to detoxification of natural foods. This study was aimed to investigate the ability of Lactobacillus acidophilus strainLA-5 in the presence and absence of yoghurt starter culture for removing Aflatoxin M1 (AFM1) in comparison with yoghurt starter cultures (108 CFU ml-1). AFM1 detoxification was evaluated for 21 days of yoghurt storage at 4°C at different concentrations of Aflatoxin (0.1, 0.5 and 0.75 µg L-1). The amounts of unbound AFM1 were determined using competitive Enzyme-Linked Immunosorbent Assay (ELISA). L. acidophilus combined with yoghurt starter culture and alone could significantly (P≤ 0.05) remove AFM1 compared to control group. The results indicated that increasing initial AFM1 concentration in the yoghurt samples and storage time affected the capacity of AFM1 binding.
外文关键词：Aflatoxin M1； Biological detoxification；Enzyme-linked immunosorbent assay；Lactic acid bacteria；Yoghurt
作者：N. Adibpour；S. Soleimanian-Zad；M. Sarabi-Jamab；等
作者单位：Isfahan University of Technology
期刊名称：JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
期刊影响因子：0.816
出版年份：2016
出版刊次：9-10
点击下载：贮藏时间与黄曲霉毒素M-1浓度对发酵乳制品中嗜酸乳杆菌毒素结合能力的影响