中文摘要：伏马菌毒素，尤其是FB1、FB2、FB3、FB4，是由有重要经济意义的霉菌毒素，通常由玉米、水稻和高粱等作物中的镰刀菌产生，或者由葡萄中的黑曲霉菌产生。本文首次报道了由曲霉属真菌千岁兰产生的两类非胺类伏马菌素（FPy and FLa）的分离与结构鉴定方法。利用浮萍生物测定技术发现，这些新化合物中的毒素明显低于伏马菌毒素B，这为伏马菌素毒理机制研究提供了新的视角。研究结果表明，黑曲霉产生的酶可以降解伏马菌素的毒性。
外文摘要：Fumonisins, notably FB1, FB2, FB3, and FB4, are economically important mycotoxins produced by a number Fusarium sp. that occur on corn, rice, and sorghum as well as by Aspergillus sp. on grapes. The fumonisin scaffold is comprised of a C18 polyketide backbone functionalized with two tricarballylic esters and an alanine derived amine. These functional groups contribute to fumonisin's ability to inhibit sphingolipid biosynthesis in animals, plants, and yeasts. We report for the first time the isolation and structure elucidation of two classes of nonaminated fumonisins (FPy and FLa) produced by Aspergillus welwitschiae. Using a Lemna minor (duckweed) bioassay, these new compounds were significantly less toxic in comparison to the fumonisin B mycotoxins, providing new insight into the mechanism of fumonisin toxicity. Time course fermentations monitoring the production of FB4, FPy4, and FLa4, as well as (13)C and (15)N stable isotope incorporation, suggest a novel postbiosynthetic oxidative deamination process for fumonisins. This pathway was further supported by a feeding study with FB1, a fumonisin not produced by Aspergillus sp., which resulted in its transformation to FPy1. This study demonstrates that Aspergillus have the ability to produce enzymes that could be used for fumonisin detoxification.
作者：Burgess, KMN;；Renaud, JB；McDowell, T；等
作者单位：Agr & Agri Food Canada
期刊名称：ACS CHEMICAL BIOLOGY
期刊影响因子：5.09
出版年份：2016
出版刊次：9
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