中文摘要：玉米赤霉烯酮（ZEA）是一种由镰刀菌产生的次生代谢物。ZEA会引发很多副作用，但是其作用机理，尤其是对十二指肠的作用机理，尚不明了。本研究利用微阵列技术对ZEA暴露后的猪十二指肠基因表达模式的变化情况进行评估。基因表达的数据经qRT-PCR和ELISA确认。基因表达数据还被外推到人类同源基因并且利用IPA方法在人类健康环境中进行数据分析。结果显示，基因表达模式受ZEA暴露影响显著。ZEA会对十二指肠产生复杂的影响。ZEA不仅能够激活免疫应答基因，而且与大肠癌的发生有关。尤其是ZEA与其它环境有毒物质或不同微生物共生时，其影响会更加显著。
外文摘要：Background：Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. ZEA was proved to exert a wide range of unwanted side effects, but its mechanism of action, particularly at duodenum levels, remains unclear. In our study based on the microarray technology we assessed the alteration of gene expression pattern Sus scrofa duodenum which has been previously exposed to ZEA. Gene expression data was validated by qRT-PCR and ELISA. The gene expression data were further extrapolated the results to their human orthologues and analyzed the data in the context of human health using IPA (Ingenuity Pathways Analysis).Results：Using Agilent microarray technology, we found that gene expression pattern was significantly affected by ZEA exposure, considering a 2-fold expression difference as a cut-off level and a p-value < 0.05. In total, we found 1576 upregulated and 2446 downregulated transcripts. About 1084 genes (764 downregulated and 751 overexpressed) were extrapolated to their human orthologues. IPA analysis showed various altered key cellular and molecular pathways. As expected, we observed a significant alteration of immune response related genes, MAPK (mitogen activate protein kinases) pathways or Toll-Like Receptors (TLRs). What captured our attention was the modulation of pathways related to the activation of early carcinogenesis.Conclusions：Our data demonstrate that ZEA has a complex effect at duodenum level. ZEA is able to activate not only the immune response related genes, but also those relate to colorectal carcinogenesis. The effects can be more dramatic when connected with the exposure to other environmental toxic agents or co-occurrence with different microorganisms.
外文关键词：Zearalenone；Duodenum；Sus scrofa；Extrapolation to human；Health；Microarray
作者：Braicu, C；Cojocneanu-Petric, R；Jurj, A；等
作者单位：Univ Med & Pharm
期刊名称：BMC GENOMICS
期刊影响因子：3.867
出版年份：2016
出版刊次：8
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