中文摘要：本研究研发了一种一次性、无标记的电化学免疫传感器，该传感器将抗AFB1抗体固定在壳聚糖-纳米金粒子(chitosan-AuNPs) 修饰金电极表面，可用于小麦中黄曲霉毒素B1（AFB1）检测。
外文摘要：A disposable and label-free electrochemical immunosensor was developed for aflatoxin B 1 (AFB 1 ) determination in wheat based on immobilization of anti-AFB 1 antibodies on the chitosan-gold nanoparticles (chitosan-AuNPs) modified gold microelectrode. The chitosan-AuNPs nanocomposite formed by one-step electrodeposition using chronoamperometry not only exhibited improved conductivity but also offered porous three-dimensional morphology, plentiful amine groups and good biocompatibility for antibody immobilization via covalent binding. To evaluate the effect of electrodeposition conditions on the surface structure and amperometric response of the chitosan-AuNPs modified microelectrode, both scanning electron microscope (SEM), cyclic voltammetry (CV) and energy dispersive spectroscopy (EDS) were studied under the different parameters of deposition potential and time. The morphology and electrochemical characterizations of the immunosensor were also investigated by atomic force microscopy (AFM) and CV. The developed immunosensor demonstrated good linearity between highly sensitive current response and AFB 1 concentration in two ranges of 0.2–202ng02mL 611 and 2–3002ng02mL 611 , with a detection limit of 0.1202ng02mL 611 (S/N02=023). For assay of AFB 1 in wheat matrix, the linear range from 1.6 to 3202ng02mL 611 was obtained considering the extraction of the sample.
外文关键词：Electrochemical immunosensor；Aflatoxin；Microelectrode；Chitosan-based nanocomposite；Gold nanoparticles；Non-competitive immunoassay
作者：Ma, Haihua；Sun, Jizhou；Zhang, Yuan；等
作者单位：中国科学院
期刊名称：BIOCHEMICAL ENGINEERING JOURNAL
期刊影响因子：2.463
出版年份：2016
出版刊次：8
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