中文摘要：本研究中对基于Fe3O4@SiO2@Au磁性纳米粒子（Au-SCMPs）的间接化学发光竞争免疫法进行了优化改进，以用于进行黄曲霉毒素B1的检测。为了提高检测效率和灵敏度，该方法结合了2',6'-dimethylcarbonylphenyl-10-sulfopropyl acridinium-9-carboxylate 4'-NHS ester (NSP-DMAE-NHS) 高效发光试剂和Au-SCMPs简化分离过程。结果表明，该检测方法的IC50和LOD值比传统ELISA法更低。该检测方法快速、成本低，适用于黄曲霉毒素B1检测。
外文摘要：An indirect competitive chemiluminescence (CL) immunoassay based on Fe3O4@SiO2@Au magnetic nanoparticles (Au-SCMPs) has been optimized and developed for the detection of aflatoxin B-1 (AFB(1)). To improve the detection sensitivity and efficiency, this method combines 2',6'-dimethylcarbonylphenyl-10-sulfopropyl acridinium-9-carboxylate 4'-NHS ester (NSP-DMAE-NHS) as a new kind of high efficient luminescence reagent with a simplified separation procedure based on the optimized Au-SCMPs. Superparamagnetic nanoparticles were coated with different sizes of Au colloids (18, 30, and 50 nm), and their capacities in immobilization of bovine serum albumin (BSA) were investigated. The BSA-AFB(1) conjugate (BSA-AFB(1)) was immobilized on the optimized Au-SCMPs and competed with the free AFB(1) for specially binding the NSP-DAME-NHS-labeled anti-AFB(1) antibody (mAb-AFB(1)). After the indirect competitive immunoreactions and magnetic separation, the CL of the nanoparticles was measured by a homemade luminescent measurement system. Under the optimum conditions, the IC50 value was 0.07 ng mL(-1) and the limit of detection (LOD) of AFB(1) was 0.01 ng mL(-1), respectively. The result shows a much lower IC50 and LOD than that typically achieved by ELISA. This proposed immunoassay method was rapid, low-cost, and suitable for the detection of AFB(1).
外文关键词：Chemiluminescence immunoassays；Aflatoxin B1；Acridinium ester；Fe3O4@SiO2@Au；magnetic nanocomposites
作者：Junfeng Li；Xiangyi Fang；Yucong Yang；等
作者单位：西安交通大学
期刊名称：Food Analytical Methods
期刊影响因子：2.167
出版年份：2016
出版刊次：9
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