中文摘要：巢状亚属（Aspergillus section Nidulantes）是一种关乎室内空气质量、食品腐败、霉菌毒素产生以及人类致病性等的重要真菌。本研究尽可能收集了大量的巢状亚属样本，并从四个位点获取NDA测序数据来对巢状亚属和其它种类真菌的系统进化情况进行分析。另外还比较了其表型性状。本研究的目标是要识别巢状亚属的单系类群及其它类群。本研究表明密码子使用特征分析方法可以很容易的区分这些类群，本研究还提供了允许benA扩增的引物选择。从欧洲的洞窟中发现了A. croceus (西班牙)和A. askiburgiensis (捷克)两种巢状菌素。胞外代谢物组分析表明，这两种巢状菌素都会产生杂色曲霉毒素及其它化合物。
外文摘要：Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard beta-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.
外文关键词：Aspergillus nidulans；Codon usage；Emericella；Mycotoxins；Paralogous genes；Subgeneric classification of Aspergillus
作者：Hubka, Vit；Novakova, Alena；Peterson, Stephen W.；等
作者单位：Charles Univ Prague
期刊名称：PLANT SYSTEMATICS AND EVOLUTION
期刊影响因子：1.361
出版年份：2016
出版刊次：11
点击下载：通过描述两种新的杂色曲霉素菌种，对巢状亚属进行再评估