中文摘要：产毒真菌曲霉属和青霉属与人类和动物健康有重要关联。为了降低对消费者健康的风险，有必要研究一种从食品和饲料中快速分离产毒真菌的方法。显色培养基和试剂的应用为本研究提供了可能性。本研究旨在利用显色培养基和试剂测试方法，从食物中快速诊断分离出的产毒真菌黄曲霉、碳黑曲霉、赭曲霉、扩展青霉、疣孢青霉和橘青霉。研究结果表明，显色培养基和试剂非常适用于微生物和真菌实验室，其优点包括48-72小时快速诊断、制备简单、容易获得和价格低廉等。
外文摘要：The aim of this study was to experimentally test chromogenic media and reagents for a rapid diagnosis of toxigenic microfungi Aspergillus flavus, A. carbonarius, A. ochraceus, Penicillium expansum, P. verrucosum, and P. citrinum isolated from foodstuffs. Toxigenic microfungi of Aspergillus and Penicillium species are considered to be of major relevance for human and animal health. The presence of these toxigenic microfungi in foodstuffs and feedstuffs raises a potential risk of mycotoxins (for example presence of aflatoxins, cyclopiazonic acid, ochratoxin A, patulin or citrinin). In order to reduce the risks for consumers’ health, it is necessary to set rapid methods for toxigenic microfungi isolated from foodstuffs and also feedstuffs. Application of chromogenic media and reagents presents one of the possibilities. The selected strains of toxigenic microfungi, producing mycotoxins, were tested on chromogenic media. The chromogenic media and reagents in our study are suitable for a microbiological and mycological laboratory. The benefits of chromogenic media and reagents include a rapid diagnosis within 48–72 h, simple preparation, easy availability and relatively low prices.
外文关键词：Toxigenic microfungi；mycotoxins；chromogenic media；reagents；food and feed safety
作者：Jefremova, M；Ostry, V；Malir, F；等
作者单位：Natl Inst Publ Hlth Prague，Czech Republic
期刊名称：ACTA VETERINARIA BRNO
期刊影响因子：0.442
出版年份：2016
出版刊次：2
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