中文摘要：本文构建了基于自组装的星形巯基乙酸修饰硫化锌量子点电化学免疫传感器并将其应用于食品中潜在毒素的检测。检测结果表明：该传感器的响应灵敏度达到176.58Ω/(ng/mL)/cm2，检测限为0.02ng/mL，检测范围为0.25-2.0 ng/mL。本文所构建的传感器对其他毒素检测生物传感器的构建具有一定的借鉴意义。
外文摘要：We report the results of studies relating to the fabrication of an electrochemical immunosensor based on self-assembled of star shaped thioglycolic acid capped zinc sulphide quantum dots (ZnS QDs) synthesized by hot injection method. Structural and morphological investigations of ZnS QDs have been accomplished via transmission electron microscopy (TEM), X-ray diffraction spectroscopy (XRD), UV-visible spectroscopy (UV-vis) and Photoluminescence spectroscopic (PL) techniques. Further, monoclonal aflatoxin-B-1 antibodies (anti-AFB(1)) have been covalently immobilized onto the ZnS/APTES/ITO electrode surface for the detection of aflatoxin-B-1, a potential food toxin using electrochemical impedance spectroscopy. The impedemetric response of the proposed immunosensor shows high sensitivity (176.58 Omega/(ng/mL)/cm2), improved detection limit (0.02 ng/mL) and wide detection range (0.25-2.0 ng/mL). The ZnS QDs based platform provides an efficient platform for the fabrication of immunosensor for the detection of aflatoxin- B-1 and these results have implications in the design of highly efficient biosensors for other potential toxins. (C) 2016 Elsevier B.V. All rights reserved.
外文关键词：Zinc sulphide；Quantum dots；Aflatoxin；Impedance
作者：Hema Bhardwaj；Chandan Singh；Manoj kumar Pandey
期刊名称：Sensors and Actuators B: Chemical
期刊影响因子：4.756
出版年份：2016
出版刊次：3
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