中文摘要：CRISPRs即成簇的规律间隔的短回文重复序列，主要由短的高度保守的DNA重复片段组成；这些片段与长度相似的间区序列间隔排列。CRISPRs这些独特的序列特征可以对包括产志贺毒素大肠杆菌在内的多种细菌进行分型。研究回顾了CRISPRs技术在食品或水中产志贺毒素大肠杆菌检测或分型中方面的应用情况。
外文摘要：Among strains of Shiga-toxin-producing Escherichia coli (STEC), seven serogroups (O26, O45, O103, O111, O121, O145, and O157) are frequently associated with severe clinical illness in humans. The development of methods for their reliable detection from complex samples such as food has been challenging thus far, and is currently based on the PCR detection of the major virulence genes stx1, stx2, and eae, and O-serogroup-specific genes. However, this approach lacks resolution. Moreover, new STEC serotypes are continuously emerging worldwide. For example, in May 2011, strains belonging to the hitherto rarely detected STEC serotype O104:H4 were identified as causative agents of one of the world's largest outbreak of disease with a high incidence of hemorrhagic colitis and hemolytic uremic syndrome in the infected patients. Discriminant typing of pathogens is crucial for epidemiological surveillance and investigations of outbreaks, and especially for tracking and tracing in case of accidental and deliberate contamination of food and water samples. Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of short, highly conserved DNA repeats separated by unique sequences of similar length. This distinctive sequence signature of CRISPRs can be used for strain typing in several bacterial species including STEC. This review discusses how CRISPRs have recently been used for STEC identification and typing.
外文关键词：STEC；E. coli；CRISPR；Detection；Typing
作者：Sabine Delannoy；Lothar Beutin；Patrick Fach
作者单位：巴黎东大学
期刊名称：Environmental Science and Pollution Research
期刊影响因子：2.76
出版年份：2016
出版刊次：9
点击下载：利用CRISPRs技术可提高对产志贺毒素大肠杆菌检测和分型的效率（综述）