中文摘要：黄曲霉能够合成聚酮类次生代谢产物包括致肝癌物质黄曲霉毒素B1。真菌的繁殖和传播主要通过产生分生孢子进行。黄曲霉还能产生坚硬的菌丝体（菌核），菌核能够适应不利的生长环境。作者研究了A. flavus fluP（次生代谢物的基因簇41的主要聚酮合酶基因）在真菌发育中的作用。研究结果表明，FluP聚酮合酶参与扩散性代谢物的合成，它作为信号分子来调节硬化起源（sclerotiogenesis）。
外文摘要：Aspergillus flavus is able to synthesize a variety of polyketide-derived secondary metabolites including the hepatocarcinogen, aflatoxin B 1 . The fungus reproduces and disseminates predominantly by production of conidia. It also produces hardened mycelial aggregates called sclerotia that are used to cope with unfavourable growth environments. In the present study, we examined the role of A. flavus fluP , the backbone polyketide synthase gene of secondary metabolite gene cluster 41, on fungal development. The A. flavus CA14 fluP deletion mutant (AfΔfluP) grew and accumulated aflatoxin normally but produced a lower amount of sclerotia than the parental strain. This was also true for the Aspergillus parasiticus BN9 fluP deletion mutant (ApΔfluP). The A. flavus fluP gene was positively regulated by developmental regulators of VeA and VelB but not by the global regulator of secondary metabolism, LaeA. Overexpression of fluP in AfΔfluP (OEfluP) elevated its ability to produce sclerotia compared to that of the parental strain. Coculture of OEfluP with CA14, AfΔfluP, ApΔfluP, or an A. flavus pptA deletion mutant incapable of producing functional polyketide synthases also allowed increased sclerotial production of the respective strains at edges where colonies made contact. Acetone extracts of OEfluP but not of AfΔfluP exhibited the same effect in promoting sclerotial production of AfΔfluP. These results suggest that FluP polyketide synthase is involved in the synthesis of a diffusible metabolite that could serve as a signal molecule to regulate sclerotiogenesis.
外文关键词：Aflatoxin；Gene cluster；Polyketide synthase；Sclerotia
作者：Chang, PK；Scharfenstein, LL；Ehrlich, KC；等
作者单位：ARS, Southern Reg Res Ctr, USDA
期刊名称：FUNGAL BIOLOGY
期刊影响因子：2.244
出版年份：2016
出版刊次：10
点击下载：黄曲霉素fluP的代谢物促进菌核产生