中文摘要：本文开发了一种基于超声辅助的DLLME分析技术提取中药中19种农药的方法。实验结果发现：不同添加样本的19种农药回收率均在81-108%之间，RSD值低于13%，定量限均在0.0075-0.81μg/kg之间，而且该方法简单快速灵敏，可用于监测传统中药中多种农药残留。
外文摘要：The objective of this study was to develop an analytical method based on ultrasonic-assisted dispersive liquid-liquid microextraction (DLLME) for the determination of 19 pesticides in Shengmaiyin and its resulting crude drugs Ophiopogon japonicas and Codonopis pilosula. The target compounds were qualitatively and quantitatively studied via ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) operated in multiple reaction-monitoring modes. The UHPLC-MS/MS conditions were optimized to complete chromatographic separation in 9 min. The correlation coefficient was higher than 0.993 for each pesticide. Average recoveries of the 19 compounds from different fortified samples ranged between 81 and 108 % with inter-RSD values below 13 %. The limits of quantification (LOQs) for 19 compounds were in the range of 0.0075-0.81 mu g kg(-1). Recovery LOQs of the DLLME method were compared with the routine QuEChERS method, and the results showed that the proposed method is very simple, rapid, and sensitive. This makes it a more applicable method for monitoring multiple classes of pesticides in traditional Chinese medicine.
外文关键词：Pesticides；Chinese traditional medicine；Ultrasonic-assisted dispersive liquid–liquid microextraction · UHPLC–MS/MS
作者：Yao Qin；Lina Chen； Xiaojing Yang
期刊名称：CHROMATOGRAPHIA
期刊影响因子：1.332
出版年份：2016
出版刊次：5
点击下载：应用DLLME和UHPLC-MS/MS方法测定中药中19种代表性农药残留