中文摘要：本研究的目的是应用DHPLC技术建立一种基于SNP的卡斯特尔韦特拉诺黑面包原料硬粒小麦品种“Timilia”的追溯方法。应用20个SNPs（8个转换位点、12个颠换位点）对DHPLC技术进行了优化，结果表明：该技术对样本中该品种含量的检测范围为2.5%-60%，是一种追踪面包中该品种原料组成的有效手段；该技术对卡斯特尔韦特拉诺黑面包的防伪认证具有重要作用。
外文摘要：DNA-based traceability is a very powerful tool to allow the identification of genetic material along the food production chain. In particular, it is able to verify the presence of a certain cultivar in order to meet the quality claims of local and certified products, or to detect any adulteration or contamination. The aim of this work was to apply the Denaturing High Performance Liquid Chromatography (DHPLC) technique for setting up a Single Nucleotide Polymorphism (SNP)-based method to achieve the varietal traceability of the durum wheat cultivar 'Timilia' in the 'Pane Nero di Castelvetrano' (Castelvetrano Black Bread) Slow Food Presidium. The DHPLC technique was optimized using 20 SNPs (8 transitions and 12 transversions) for both the starting whole meals and the corresponding breads, giving comparable results represented by an amount of detectable Timilia cv. ranging from 2.5% to 60% or more, thus providing an efficient tool to trace such cultivar in bread claimed to be Pane Nero di Castelvetrano. The obtained results represent the first application of DHPLC in the field of food science, and will help to preserve the authenticity of Pane Nero di Castelvetrano.
外文关键词：Pane Nero di Castelvetrano；DHPLC;SNP；Varietal traceability；Durum wheat
作者：Giancaspro, Angelica1；Colasuonno, Pasqualina1；Zito, Daniela1等
作者单位：意大利巴里大学
期刊名称：Food Control
期刊影响因子：3.388
出版年份：2015
出版刊次：7
点击下载：基于SNPs位点、应用DHPLC技术建立一种卡斯特尔韦特拉诺黑面包原料品种追溯方法