表达杀虫Cry1Ab/1Ac基因的转基因棉GK 12可以有效控制农田的鳞翅目害虫，但它可能对土壤的真菌群落产生意想不到的副作用。本文在密集常规种植系统内，基于各生长阶段和三年的数据，用实时聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）方法对转Bt基因棉花和非转Bt基因棉花根际土壤中真菌群落的规模和结构进行了分析。研究发现，在所有生长阶段中，转基因棉GK 12与其近等基因系的，根际土壤的真菌群落规模和结构均无明显差异，但在某些生长阶段，转基因棉GK 12与常规棉DP 5415相比有所不同。真菌群落的规模和结构受年度和生长阶段变化的影响显著。DGGE的测序结果发现，转基因棉GK 12与其近等基因系的主要真菌没有差别。研究结果表明，在连续三年时间内，转基因棉GK 12对根际土壤中真菌群落的规模和结构没有显著影响。
外文摘要：
Transgenic cotton GK 12 expressing insecticidal Cry1Ab/1Ac gene can effectively control the lepidopteran pests in agriculture, however it may have unintended side-effects on soil fungal communities. Therefore, the fungal community size and structure in rhizosphere soil of Bt and non-Bt cottons were analyzed by real-time polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE) at growth stages and three annual replications in an intensive conventional cultivation system. The Cry1Ab/1Ac protein was undetectable or occasionally detectable at a low level (<0.1 ng/g soil) at seedling and senescence, and that the concentration of Cry1Ab/Ac protein in soil extracts did not increase over time or repeated cultivations. No significant differences in fungal population size and structure in rhizosphere soil were found between Bt-cotton GK 12 and its near-isogenic comparator at all growth stages, but they were different between Bt-cotton GK 12 and conventional cotton DP 5415 at some growth stages. The fungal population size and structure was significantly influenced by variation in years and plant growth stages. Sequencing results from DGGE bands showed that the dominant fungi were not different between Bt-cotton GK 12 and its near-isogenic comparator. Thus, the data of this study do not indicate any significant effect of Bt-cotton GK 12 on the fungal community size and structure in rhizosphere soil during these three continuous years. (C) 2016 Elsevier Masson SAS. All rights reserved.
外文关键词：Bt crops；Soil fungi；Risk assessment；Molecular ecology
作者：Xie, Ming；Zhang, Yan-Jun；Peng, De-Liang；等
作者单位：中国农业科学院
期刊名称：EUROPEAN JOURNAL OF SOIL BIOLOGY
期刊影响因子：1.951
出版年份：2016
出版刊次：3
点击下载：田间试验表明，转Cry1Ab/Ac基因棉花的种植对根际土壤中真菌群落的结构没有显著影响