本文研究的目的是探讨在喂养过转基因大豆粉的肉兔食糜和组织中是否存在转基因DNA。作者将十五只雄性新西兰大白兔分为两组，其中十只喂养含有10%转基因大豆粉的复合饲料，五只喂养含有常规大豆粉的复合饲料。论文使用特异性基因组DNA试剂盒进行了DNA提取，并采用实时PCR对所有样品的转基因抗农达大豆40-3-2和内源性凝集素基因的寡核苷酸引物和探针的特异性进行了监测。研究发现，除了从食糜、粪便和头发中提取的DNA外，喂养转基因大豆的兔子组织DNA样本中没有发现tDNA片段；在全部兔子的组织中均检测到了ACTB基因。兔子组织中没有发现大豆tDNA是一个重要发现，这表明喂养转基因大豆兔子的肉与喂养常规作物兔子的肉是相同的；而在胃食糜和粪便中出现重组DNA，表明兔子胃肠道中的大豆DNA没有完全消化，而兔子头发上存在的微量大豆转基因则表明存在环境污染。
外文摘要：
Numerous animal feeding studies have investigated the presence of DNA from transgenic plants in tissues from different animal species, but the data reported are sometimes controversial. The aim of this study was to investigate the presence of transgenic DNA (tDNA) in the digesta and tissues of a meat rabbit breed fed genetically modified (GM) soybean meal. Fifteen male New Zealand White rabbits were used for the experimental trial. Ten rabbits (treated group [TG]) were fed a mixed feed containing 10% GM soybean meal and 5 rabbits (control group [CG]) received a mixed feed containing conventional soybean meal, both from weaning (28 d of age) to slaughter (80 +/- 3 d). Samples of blood, liver, kidney, heart, stomach, intestine (jejunum), lateral quadricep muscle, longissimus muscle, and perirenal adipose tissue were collected to assess the possible DNA transfer from GM feed to animal tissues. Samples of stomach contents and feces were also taken to study the degradability of ingested tDNA from feed in the digestive tract of rabbit. Moreover, samples of hair were collected to determine the possible environmental contamination from feed powders present on the farm. The DNA extraction was performed using specific genomic DNA kits. All samples were monitored, by using real-time PCR, for oligonucleotide primers and probes specific for the transgenic Roundup Ready soybean 40-3-2 and for the endogenous lectin (LE1) gene. As an internal control of rabbit tissues, the presence of the beta-actin (ACTB) gene was used. In this study, no fragments of tDNA were detectable in tissue DNA samples of rabbits except in the extracted DNA from stomach digesta, feces, and hair of rabbits fed with GM soybean. Similar results were found for the reference LE1 gene, whereas the presence of the ACTB gene was detected in all rabbit tissues. The lack of tDNA of soybean in rabbit tissues represents an important result, which demonstrates that meat from rabbits fed a diet containing GM feed is as that derived from rabbits fed conventional crops. The recombinant DNA recovered in the stomach digesta and in feces indicates an incomplete digestion of the soybean DNA in the gastrointestinal tract of the rabbit, whereas the presence of trace soybean transgene in the hair of the TG rabbits is suggestive of an environmental contamination.
外文关键词：digesta；genetically modified soybean；rabbit；real-time polymerase chain reaction；tissues；transgenic deoxyribonucleic acid
作者：Morera, P.；Basirico, L.；Ronchi, B.；等
作者单位：图西亚大学
期刊名称：JOURNAL OF ANIMAL SCIENCE
期刊影响因子：2.014
出版年份：2016
出版刊次：3
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