近年来，全球大米供给已被未经批准的转基因水稻品种所污染。更快、更有效的转基因污染检测方法可以防止掺假的食品、饲料和种子继续消费，还可以减少潜在的环境、健康或经济损失。本文用品系特异性片段研发了一个简单可靠、具有成本效益的多重聚合酶链反应（PCR）方法，该方法可用来检测KMD1和KF6水稻，且在该方法中每个品系的检测限约为0.1%。研究发现，本文开发的多重PCR检测方法可以快速、同步检测转基因水稻。
外文摘要：
Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice.
外文关键词：multiplex polymerase chain reaction；genetically modified rice；detection；event-specific
作者：Lu, J.；Ji, G. Z.；Li, G.；等
作者单位：沈阳农业大学
期刊名称：CEREAL RESEARCH COMMUNICATIONS
期刊影响因子：0.528
出版年份：2016
出版刊次：3