转Cry1Ac基因甘蔗为防治螟害提供了一个有效方法。土壤微生物在养分转化和元素循环中有重要的作用，然而目前几乎未见相关报道，因此急需对转Cry1Ac基因甘蔗土壤生态系统的生物安全性进行评价。本文旨在探讨转Cry1Ac基因甘蔗对根际土壤酶活性及微生物群落多样性的潜在影响，并探讨是否有基因水平转移的基因流出现。研究发现，未见从转Cry1Ac基因甘蔗到土壤的水平基因流；非转基因和转Cry1Ac基因甘蔗间的可培养微生物数量无显著差异，且不同甘蔗品种和生长阶段间无显著的交互作用。非转基因和转Cry1Ac基因甘蔗间的生物群落水平有相对一致的趋势，且土壤酶活性无显著差异。研究结果表明，转Cry1Ac基因甘蔗不影响微生物群落的结构和根际的功能多样性，但对土壤酶有一些负面影响。
外文摘要：
Cry1Ac transgenic sugarcane provides a promising way to control stem-borer pests. Biosafety assessment of soil ecosystem for cry1Ac transgenic sugarcane is urgently needed because of the important role of soil microorganisms in nutrient transformations and element cycling, however little is known. This study aimed to explore the potential impact of cry1Ac transgenic sugarcane on rhizosphere soil enzyme activities and microbial community diversity, and also to investigate whether the gene flow occurs through horizontal gene transfer. We found no horizontal gene flow from cry1Ac sugarcane to soil. No significant difference in the population of culturable microorganisms between the non-GM and cry1Ac transgenic sugarcane was observed, and there were no significant interactions between the sugarcane lines and the growth stages. A relatively consistent trend at community-level, represented by the functional diversity index, was found between the cry1Ac sugarcane and the non-transgenic lines. Most soil samples showed no significant difference in the activities of four soil enzymes: urease, protease, sucrose, and acid phosphate monoester between the non-transgenic and cry1Ac sugarcane lines. We conclude, based on one crop season, that the cry1Ac sugarcane lines may not affect the microbial community structure and functional diversity of the rhizosphere soil and have few negative effects on soil enzymes.
外文关键词：cry1Ac transgenic sugarcane；risk assessment；gene flow；enzyme activity；functional diversity；community structure
作者：Dinggang Zhou； Liping Xu； Shiwu Gao；等
作者单位：福建农林大学
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.495
出版年份：2016
出版刊次：3
点击下载：转Cry1Ac基因甘蔗不影响微生物群落的多样性，且对根际土壤酶活性没有明显的影响