相比单一实时PCR，多重实时PCR的优点是节省时间和资源。本文使用基TaqMan (R)的双重和三重实时PCR检测Bt11, Bt176 和MON89034三种转基因玉米的品系特异性序列，并开发特定的内源性Adh1基因来同时识别多个品系。作者基于TaqMan (R)的双重实时PCR方法检测Bt176品系特异性序列和Adh1，也可起到优化量化的目的。本研究方法的检测限达到0.05%，根据已有报导的基于实时PCR的TaqMan定量限达到0.5%。
外文摘要：
Multiplexing in real-time PCR offers advantages over singleplex real-time PCR by saving time and resources. SYBR (R) Green I-based duplex and triplex real-time PCR assays targeting event-specific sequences of three genetically modified (GM) maize events, namely Bt11, Bt176 and MON89034, and taxon-specific endogenous Adh1 gene were developed to simultaneously identify multiple events. Duplex real-time PCR assay based on TaqMan (R) chemistry targeting Bt176 event-specific sequence and Adh1 was also optimized for quantification purpose. Limit of detection of developed assays was up to 0.05% and limit of quantification of the reported TaqMan (R) based real-time PCR was up to 0.5%.
外文关键词：Dyes；genetically modified maize；GM detection and quantification；multiplex real-time PCR；primers and probes
作者：Bhoge, Rajesh K.；Chhabra, Rashmi；Singh, Monika；等
作者单位：Indian Council Agr Res
期刊名称：CURRENT SCIENCE
期刊影响因子：0.967
出版年份：2016
出版刊次：4
点击下载：在多重实时PCR中，使用SYBR (R) Green I和TaqMan (R)来检测和量化转基因玉米品系